Yeast Telomerase RNA Flexibly Scaffolds Protein Subunits: Results and Repercussions

David C. Zappulla

doi:10.3390/molecules25122750

Yeast Telomerase RNA Flexibly Scaffolds Protein Subunits: Results and Repercussions

David C. Zappulla

Research output: Contribution to journal › Review article › peer-review

2 Scopus citations

Abstract

It is said that "hindsight is 20-20," so, given the current year, it is an opportune time to review and learn from experiences studying long noncoding RNAs. Investigation of the Saccharomyces cerevisiae telomerase RNA, TLC1, has unveiled striking flexibility in terms of both structural and functional features. Results support the "flexible sca_old" hypothesis for this 1157-nt telomerase RNA. This model describes TLC1 acting as a tether for holoenzyme protein subunits, and it also may apply to a plethora of RNAs beyond telomerase, such as types of lncRNAs. In this short perspective review, I summarize findings from studying the large yeast telomerase ribonucleoprotein (RNP) complex in the hope that this hindsight will sharpen foresight as so many of us seek to mechanistically understand noncoding RNA molecules from vast transcriptomes.

Original language	English (US)
Article number	2750
Journal	Molecules
Volume	25
Issue number	12
DOIs	https://doi.org/10.3390/molecules25122750
State	Published - Jun 2020
Externally published	Yes

Keywords

Flexible scaffold
LncRNA
NcRNA
Noncoding RNA
RNA
Rnp
Scaffold
Senescence
Telomerase
Telomere
Tlc1

ASJC Scopus subject areas

Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry

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10.3390/molecules25122750

Cite this

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title = "Yeast Telomerase RNA Flexibly Scaffolds Protein Subunits: Results and Repercussions",

abstract = "It is said that {"}hindsight is 20-20,{"} so, given the current year, it is an opportune time to review and learn from experiences studying long noncoding RNAs. Investigation of the Saccharomyces cerevisiae telomerase RNA, TLC1, has unveiled striking flexibility in terms of both structural and functional features. Results support the {"}flexible sca_old{"} hypothesis for this 1157-nt telomerase RNA. This model describes TLC1 acting as a tether for holoenzyme protein subunits, and it also may apply to a plethora of RNAs beyond telomerase, such as types of lncRNAs. In this short perspective review, I summarize findings from studying the large yeast telomerase ribonucleoprotein (RNP) complex in the hope that this hindsight will sharpen foresight as so many of us seek to mechanistically understand noncoding RNA molecules from vast transcriptomes.",

keywords = "Flexible scaffold, LncRNA, NcRNA, Noncoding RNA, RNA, Rnp, Scaffold, Senescence, Telomerase, Telomere, Tlc1",

author = "Zappulla, {David C.}",

note = "Funding Information: Funding: I acknowledge current research support from the National Institute of General Medical Sciences of the National Institutes of Health under award number R01 GM118757 as well as completed support under K99/R00 GM080400 and from March of Dimes Basil O{\textquoteright}Connor Starter Scholar Award #5-FY12-91. Publisher Copyright: {\textcopyright} 2020 MDPI AG. All rights reserved.",

year = "2020",

month = jun,

doi = "10.3390/molecules25122750",

language = "English (US)",

volume = "25",

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T2 - Results and Repercussions

AU - Zappulla, David C.

N1 - Funding Information: Funding: I acknowledge current research support from the National Institute of General Medical Sciences of the National Institutes of Health under award number R01 GM118757 as well as completed support under K99/R00 GM080400 and from March of Dimes Basil O’Connor Starter Scholar Award #5-FY12-91. Publisher Copyright: © 2020 MDPI AG. All rights reserved.

PY - 2020/6

Y1 - 2020/6

N2 - It is said that "hindsight is 20-20," so, given the current year, it is an opportune time to review and learn from experiences studying long noncoding RNAs. Investigation of the Saccharomyces cerevisiae telomerase RNA, TLC1, has unveiled striking flexibility in terms of both structural and functional features. Results support the "flexible sca_old" hypothesis for this 1157-nt telomerase RNA. This model describes TLC1 acting as a tether for holoenzyme protein subunits, and it also may apply to a plethora of RNAs beyond telomerase, such as types of lncRNAs. In this short perspective review, I summarize findings from studying the large yeast telomerase ribonucleoprotein (RNP) complex in the hope that this hindsight will sharpen foresight as so many of us seek to mechanistically understand noncoding RNA molecules from vast transcriptomes.

AB - It is said that "hindsight is 20-20," so, given the current year, it is an opportune time to review and learn from experiences studying long noncoding RNAs. Investigation of the Saccharomyces cerevisiae telomerase RNA, TLC1, has unveiled striking flexibility in terms of both structural and functional features. Results support the "flexible sca_old" hypothesis for this 1157-nt telomerase RNA. This model describes TLC1 acting as a tether for holoenzyme protein subunits, and it also may apply to a plethora of RNAs beyond telomerase, such as types of lncRNAs. In this short perspective review, I summarize findings from studying the large yeast telomerase ribonucleoprotein (RNP) complex in the hope that this hindsight will sharpen foresight as so many of us seek to mechanistically understand noncoding RNA molecules from vast transcriptomes.

KW - Flexible scaffold

KW - LncRNA

KW - NcRNA

KW - Noncoding RNA

KW - RNA

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KW - Scaffold

KW - Senescence

KW - Telomerase

KW - Telomere

KW - Tlc1

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DO - 10.3390/molecules25122750

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Yeast Telomerase RNA Flexibly Scaffolds Protein Subunits: Results and Repercussions

Abstract

Keywords

ASJC Scopus subject areas

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