YAP and TAZ regulate cell volume

Nicolas A. Perez-Gonzalez, Nash D. Rochman, Kai Yao, Jiaxiang Tao, Minh Tam Tran Le, Shannon Flanary, Lucia Sablich, Ben Toler, Eliana Crentsil, Felipe Takaesu, Bram Lambrus, Jessie Huang, Vivian Fu, Pragati Chengappa, Tia M. Jones, Andrew J. Holland, Steven An, Denis Wirtz, Ryan J. Petrie, Kun Liang GuanSean X. Sun

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


How mammalian cells regulate their physical size is currently poorly understood, in part due to the difficulty in accurately quantifying cell volume in a high-throughput manner. Here, using the fluorescence exclusion method, we demonstrate that the mechanosensitive transcriptional regulators YAP (Yes-associated protein) and TAZ (transcriptional coactivator with PDZbinding motif) are regulators of single-cell volume. The role of YAP/TAZ in volume regulation must go beyond its influence on total cell cycle duration or cell shape to explain the observed changes in volume. Moreover, for our experimental conditions, volume regulation by YAP/TAZ is independent of mTOR. Instead, we find that YAP/TAZ directly impacts the cell division volume, and YAP is involved in regulating intracellular cytoplasmic pressure. Based on the idea that YAP/TAZ is a mechanosensor, we find that inhibitingmyosin assembly and cell tension slows cell cycle progression fromG1 to S. These results suggest that YAP/TAZ may be modulating cell volume in combination with cytoskeletal tension during cell cycle progression.

Original languageEnglish (US)
Pages (from-to)3472-3488
Number of pages17
JournalJournal of Cell Biology
Issue number10
StatePublished - Oct 7 2019

ASJC Scopus subject areas

  • Cell Biology


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