WNT16 induces proliferation and osteogenic differentiation of human perivascular stem cells

Carolyn A. Meyers; Jia Shen; Amy Lu; Aaron W. James

doi:10.1016/j.jor.2018.08.021

WNT16 induces proliferation and osteogenic differentiation of human perivascular stem cells

Carolyn A. Meyers, Jia Shen, Amy Lu, Aaron W. James

School of Medicine

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Perivascular stem cells (PSC) are a progenitor population defined by their perivascular residence. Recent studies have examined the relative difference in Wnt ligands to induce PSC differentiation, including Wnt16. Here, we examine the role of Wnt16 in the proliferation and osteogenic differentiation of human PSC. Treatment of PSC with WNT16 significantly increased cell proliferation to a greater extent than did WNT3A. In addition, WNT16 showed a significant increase in osteogenic gene expression among PSC. These data demonstrate that WNT16 represents a combined mitogenic/pro-osteogenic stimulus that may play a functional role in human mesenchymal stem cell mediated bone repair.

Original language	English (US)
Pages (from-to)	854-857
Number of pages	4
Journal	Journal of Orthopaedics
Volume	15
Issue number	3
DOIs	https://doi.org/10.1016/j.jor.2018.08.021
State	Published - Sep 2018

Keywords

DKK1
Osteogenesis
PSC
Perivascular stem cell
WNT3A
Wnt signaling

ASJC Scopus subject areas

Orthopedics and Sports Medicine

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10.1016/j.jor.2018.08.021

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title = "WNT16 induces proliferation and osteogenic differentiation of human perivascular stem cells",

abstract = "Perivascular stem cells (PSC) are a progenitor population defined by their perivascular residence. Recent studies have examined the relative difference in Wnt ligands to induce PSC differentiation, including Wnt16. Here, we examine the role of Wnt16 in the proliferation and osteogenic differentiation of human PSC. Treatment of PSC with WNT16 significantly increased cell proliferation to a greater extent than did WNT3A. In addition, WNT16 showed a significant increase in osteogenic gene expression among PSC. These data demonstrate that WNT16 represents a combined mitogenic/pro-osteogenic stimulus that may play a functional role in human mesenchymal stem cell mediated bone repair.",

keywords = "DKK1, Osteogenesis, PSC, Perivascular stem cell, WNT3A, Wnt signaling",

author = "Meyers, {Carolyn A.} and Jia Shen and Amy Lu and James, {Aaron W.}",

note = "Funding Information: The present work was supported by the NIH/NIAMS ( R01 AR070773 , K08 AR068316 ), NIH/NIDCR ( R21 DE027922 ), Department of Defense ( W81XWH-18-1-0121 , W81XWH-18-1-0336 ), American Cancer Society (Research Scholar Grant, RSG-18-027-01-CSM ), the Maryland Stem Cell Research Foundation , and the Musculoskeletal Transplant Foundation . The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of Health or Department of Defense . Publisher Copyright: {\textcopyright} 2018 Prof. PK Surendran Memorial Education Foundation",

year = "2018",

month = sep,

doi = "10.1016/j.jor.2018.08.021",

language = "English (US)",

volume = "15",

pages = "854--857",

journal = "Journal of Orthopaedics",

issn = "0972-978X",

publisher = "Elsevier BV",

number = "3",

}

TY - JOUR

T1 - WNT16 induces proliferation and osteogenic differentiation of human perivascular stem cells

AU - Meyers, Carolyn A.

AU - Shen, Jia

AU - Lu, Amy

AU - James, Aaron W.

N1 - Funding Information: The present work was supported by the NIH/NIAMS ( R01 AR070773 , K08 AR068316 ), NIH/NIDCR ( R21 DE027922 ), Department of Defense ( W81XWH-18-1-0121 , W81XWH-18-1-0336 ), American Cancer Society (Research Scholar Grant, RSG-18-027-01-CSM ), the Maryland Stem Cell Research Foundation , and the Musculoskeletal Transplant Foundation . The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of Health or Department of Defense . Publisher Copyright: © 2018 Prof. PK Surendran Memorial Education Foundation

PY - 2018/9

Y1 - 2018/9

N2 - Perivascular stem cells (PSC) are a progenitor population defined by their perivascular residence. Recent studies have examined the relative difference in Wnt ligands to induce PSC differentiation, including Wnt16. Here, we examine the role of Wnt16 in the proliferation and osteogenic differentiation of human PSC. Treatment of PSC with WNT16 significantly increased cell proliferation to a greater extent than did WNT3A. In addition, WNT16 showed a significant increase in osteogenic gene expression among PSC. These data demonstrate that WNT16 represents a combined mitogenic/pro-osteogenic stimulus that may play a functional role in human mesenchymal stem cell mediated bone repair.

AB - Perivascular stem cells (PSC) are a progenitor population defined by their perivascular residence. Recent studies have examined the relative difference in Wnt ligands to induce PSC differentiation, including Wnt16. Here, we examine the role of Wnt16 in the proliferation and osteogenic differentiation of human PSC. Treatment of PSC with WNT16 significantly increased cell proliferation to a greater extent than did WNT3A. In addition, WNT16 showed a significant increase in osteogenic gene expression among PSC. These data demonstrate that WNT16 represents a combined mitogenic/pro-osteogenic stimulus that may play a functional role in human mesenchymal stem cell mediated bone repair.

KW - DKK1

KW - Osteogenesis

KW - PSC

KW - Perivascular stem cell

KW - WNT3A

KW - Wnt signaling

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DO - 10.1016/j.jor.2018.08.021

M3 - Article

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AN - SCOPUS:85051789268

SN - 0972-978X

VL - 15

SP - 854

EP - 857

JO - Journal of Orthopaedics

JF - Journal of Orthopaedics

IS - 3

ER -

WNT16 induces proliferation and osteogenic differentiation of human perivascular stem cells

Abstract

Keywords

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