Whole-genome random sequencing and assembly of Haemophilus influenzae Rd

Robert D. Fleischmann, Mark D. Adams, Owen White, Rebecca A. Clayton, Ewen F. Kirkness, Anthony R. Kerlavage, Carol J. Bult, Jean Francois Tomb, Brian A. Dougherty, Joseph M. Merrick, Keith McKenney, Granger Sutton, Will FitzHugh, Chris Fields, Jeannine D. Gocayne, John Scott, Robert Shirley, Li Ing Liu, Anna Glodek, Jenny M. KelleyJanice F. Weidman, Cheryl A. Phillips, Tracy Spriggs, Eva Hedblom, Matthew D. Cotton, Teresa R. Utterback, Michael C. Hanna, David T. Nguyen, Deborah M. Saudek, Rhonda C. Brandon, Leah D. Fine, Janice L. Fritchman, Joyce L. Fuhrmann, N. S.M. Geoghagen, Cheryl L. Gnehm, Lisa A. McDonald, Keith V. Small, Claire M. Fraser, Hamilton O. Smith, J. Craig Venter

Research output: Contribution to journalArticlepeer-review

Abstract

An approach for genome analysis based on sequencing and assembly of unselected pieces of DNA from the whole chromosome has been applied to obtain the complete nucleotide sequence (1,830,137 base pairs) of the genome from the bacterium Haemophilus influenzae Rd. This approach eliminates the need for initial mapping efforts and is therefore applicable to the vast array of microbial species for which genome maps are unavailable. The H. influenzae Rd genome sequence (Genome Sequence DataBase accession number L42023) represents the only complete genome sequence from a free-living organism.

Original languageEnglish (US)
Pages (from-to)496-512
Number of pages17
JournalScience
Volume269
Issue number5223
DOIs
StatePublished - 1995

ASJC Scopus subject areas

  • General

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