TY - JOUR
T1 - White paper on guidelines concerning enteric nervous system stem cell therapy for enteric neuropathies
AU - Burns, Alan J.
AU - Goldstein, Allan M.
AU - Newgreen, Donald F.
AU - Stamp, Lincon
AU - Schäfer, Karl Herbert
AU - Metzger, Marco
AU - Hotta, Ryo
AU - Young, Heather M.
AU - Andrews, Peter W.
AU - Thapar, Nikhil
AU - Belkind-Gerson, Jaime
AU - Bondurand, Nadege
AU - Bornstein, Joel C.
AU - Chan, Wood Yee
AU - Cheah, Kathryn
AU - Gershon, Michael D.
AU - Heuckeroth, Robert O.
AU - Hofstra, Robert M.W.
AU - Just, Lothar
AU - Kapur, Raj P.
AU - King, Sebastian K.
AU - McCann, Conor J.
AU - Nagy, Nandor
AU - Ngan, Elly
AU - Obermayr, Florian
AU - Pachnis, Vassilis
AU - Pasricha, Pankaj J.
AU - Sham, Mai Har
AU - Tam, Paul
AU - Vanden Berghe, Pieter
N1 - Funding Information:
The work of AB and NT was supported by the National Institute for Health Research Biomedical Research Centre at Great Ormond Street Hospital for Children NHS Foundation Trust and University College London. Peter Andrews is supported by the UK Medical Research Council (MRC) (Grant no. MR/L12537/1) . Wood Yee Chan’s work was supported by grants from the Health and Medical Research Fund (Project Reference: 01120456 ), RGC General Research Fund (Project Reference: 14102214 ), The Chinese University of Hong Kong (CUHK) Direct Grant for Research (Reference no.: 2014.1.074 ) and RGC Theme-based Research Scheme (Project Reference: T12C-714/14-R ). Allan Goldstein is supported by the National Institutes of Health ( R01DK103785 ). Ryo Hotta is supported by American Neurogastroenterology and Motility Society Research Grants Program (Grant no. 2016D000927) . Sebastian King is the grateful recipient of a Career Development Award ( Murdoch Childrens Research Institute ) and the Senior Lecturer Fellowship ( Royal Australasian College of Surgeons ).
Publisher Copyright:
© 2016 The Authors
PY - 2016/9/15
Y1 - 2016/9/15
N2 - Over the last 20 years, there has been increasing focus on the development of novel stem cell based therapies for the treatment of disorders and diseases affecting the enteric nervous system (ENS) of the gastrointestinal tract (so-called enteric neuropathies). Here, the idea is that ENS progenitor/stem cells could be transplanted into the gut wall to replace the damaged or absent neurons and glia of the ENS. This White Paper sets out experts’ views on the commonly used methods and approaches to identify, isolate, purify, expand and optimize ENS stem cells, transplant them into the bowel, and assess transplant success, including restoration of gut function. We also highlight obstacles that must be overcome in order to progress from successful preclinical studies in animal models to ENS stem cell therapies in the clinic.
AB - Over the last 20 years, there has been increasing focus on the development of novel stem cell based therapies for the treatment of disorders and diseases affecting the enteric nervous system (ENS) of the gastrointestinal tract (so-called enteric neuropathies). Here, the idea is that ENS progenitor/stem cells could be transplanted into the gut wall to replace the damaged or absent neurons and glia of the ENS. This White Paper sets out experts’ views on the commonly used methods and approaches to identify, isolate, purify, expand and optimize ENS stem cells, transplant them into the bowel, and assess transplant success, including restoration of gut function. We also highlight obstacles that must be overcome in order to progress from successful preclinical studies in animal models to ENS stem cell therapies in the clinic.
KW - Cell replacement therapy
KW - Enteric nervous system
KW - Enteric neuropathies
KW - Hirschsprung disease
KW - Stem cells
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U2 - 10.1016/j.ydbio.2016.04.001
DO - 10.1016/j.ydbio.2016.04.001
M3 - Review article
C2 - 27059883
AN - SCOPUS:84964222425
SN - 0012-1606
VL - 417
SP - 229
EP - 251
JO - Developmental biology
JF - Developmental biology
IS - 2
ER -