Vitreous Fluorophotometry for Clinical Research: II. Method of Data Acquisition and Processing

Ran C. Zeimer; Norman P. Blair; Jose G. Cunha-Vaz

doi:10.1001/archopht.1983.01040020759020

Vitreous Fluorophotometry for Clinical Research: II. Method of Data Acquisition and Processing

Ran C. Zeimer, Norman P. Blair, Jose G. Cunha-Vaz

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

Several methods of data analysis permit improved evaluation of the blood-retinal barrier (BRB) by vitreous fluorophotometry. With the use of an algorithm for artifact correction, tested in vivo in situations in which large artifacts are expected, artifacts were reduced to less than the equivalent of 1 ng/mL in normal eyes 3 mm from the chorioretinal and lens peaks. A procedure developed to evaluate the lens transmittance was found to have a 12% reproducibility. In addition, the anterior and posterior sources of leakage could be separated by choosing the appropriate time of measurement after injection of the dye. Moreover, a procedure to estimate quantitatively the inward permeability of the BRB yielded a preliminary estimate of 7.2 × 10-8 cm/snornor-subjects.

Original language	English (US)
Pages (from-to)	1757-1761
Number of pages	5
Journal	Archives of ophthalmology
Volume	101
Issue number	11
DOIs	https://doi.org/10.1001/archopht.1983.01040020759020
State	Published - Nov 1983
Externally published	Yes

ASJC Scopus subject areas

Ophthalmology

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10.1001/archopht.1983.01040020759020

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abstract = "Several methods of data analysis permit improved evaluation of the blood-retinal barrier (BRB) by vitreous fluorophotometry. With the use of an algorithm for artifact correction, tested in vivo in situations in which large artifacts are expected, artifacts were reduced to less than the equivalent of 1 ng/mL in normal eyes 3 mm from the chorioretinal and lens peaks. A procedure developed to evaluate the lens transmittance was found to have a 12% reproducibility. In addition, the anterior and posterior sources of leakage could be separated by choosing the appropriate time of measurement after injection of the dye. Moreover, a procedure to estimate quantitatively the inward permeability of the BRB yielded a preliminary estimate of 7.2 × 10-8 cm/snornor-subjects.",

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AU - Blair, Norman P.

AU - Cunha-Vaz, Jose G.

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AB - Several methods of data analysis permit improved evaluation of the blood-retinal barrier (BRB) by vitreous fluorophotometry. With the use of an algorithm for artifact correction, tested in vivo in situations in which large artifacts are expected, artifacts were reduced to less than the equivalent of 1 ng/mL in normal eyes 3 mm from the chorioretinal and lens peaks. A procedure developed to evaluate the lens transmittance was found to have a 12% reproducibility. In addition, the anterior and posterior sources of leakage could be separated by choosing the appropriate time of measurement after injection of the dye. Moreover, a procedure to estimate quantitatively the inward permeability of the BRB yielded a preliminary estimate of 7.2 × 10-8 cm/snornor-subjects.

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Vitreous Fluorophotometry for Clinical Research: II. Method of Data Acquisition and Processing

Abstract

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