TY - JOUR
T1 - Vitamin A-dependent fucose-glycopeptide from rat tracheal epithelium
AU - Bonanni, Fabrizio
AU - De Luca, Luigi
N1 - Funding Information:
We thank Professor George Wolf, in whose laboratory part of this investigation was carried out for advice and constant help throughout this work. This work was supported, in part, by National Institutes of Health grant AM-08732.
PY - 1974/5/24
Y1 - 1974/5/24
N2 - Tracheas from normal and vitamin A-deficient rats were incubated in the presence of l-[3H]fucose and d-[14C]glucosamine to label epithelial and mesenchymal glycoproteins. The epithelium and the cartilage were separated by incubation with testicular hyraluronidase and processed separately for the preparation of glycopeptides. One major epithelial glycopeptide was doubly labeled. In vitamin A deficiency the amount of l-[3H]fucose was reduced to 33% that of the normal. The tracheal fucose-glycopeptide, in molar ratios, contained fucose 1.0, mannonse 1.0, galactose 0.4, hexasamine 4.7, and sialic acid 3.5. Sulfate was absent.
AB - Tracheas from normal and vitamin A-deficient rats were incubated in the presence of l-[3H]fucose and d-[14C]glucosamine to label epithelial and mesenchymal glycoproteins. The epithelium and the cartilage were separated by incubation with testicular hyraluronidase and processed separately for the preparation of glycopeptides. One major epithelial glycopeptide was doubly labeled. In vitamin A deficiency the amount of l-[3H]fucose was reduced to 33% that of the normal. The tracheal fucose-glycopeptide, in molar ratios, contained fucose 1.0, mannonse 1.0, galactose 0.4, hexasamine 4.7, and sialic acid 3.5. Sulfate was absent.
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U2 - 10.1016/0304-4165(74)90282-7
DO - 10.1016/0304-4165(74)90282-7
M3 - Article
C2 - 4407036
AN - SCOPUS:0016200318
SN - 0304-4165
VL - 343
SP - 632
EP - 637
JO - BBA - General Subjects
JF - BBA - General Subjects
IS - 3
ER -