Vitamin a contents of rat intestinal epithelium and jejunal mucinous adenocarcinoma

P. Ramnathan Sundaresan; Luigi M. De Luca

doi:10.1093/jnci/58.6.1643

Vitamin a contents of rat intestinal epithelium and jejunal mucinous adenocarcinoma

P. Ramnathan Sundaresan, Luigi M. De Luca

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Abstract

Concentrations of retinol and retinyl esters were assayed in rat intestinal mucosa and in chemically induced transplanted mucinous adenocarcinoma of the jejunum. Lipid extract from the tissues was chromatographed on deactivated alumina to isolate retinol and retinyl esters, which were determined by specific spectrofluorometry. Normal intestinal mucosa tissue contains 556 ng of retinol equivalents as retinyl esters and 303 ng of free retinol/g of wet tissue. The concentration of retinyl esters in the intestinal mucosa from rats carrying the transplanted tumor was 341 ng/g wet tissue; no free retinol was detected in the small intestinal epithelium of these rats. Liver tissue from the tumorbearing rats contained 157 μg of retinol equivalents as retinyl esters and 136 μg of free retinol/g of wet tissue. The concentration of vitamin A per cell in the adenocarcinoma tissue was about 20 times less than that in intestinal epithelium.

Original language	English (US)
Pages (from-to)	1643-1645
Number of pages	3
Journal	Journal of the National Cancer Institute
Volume	58
Issue number	6
DOIs	https://doi.org/10.1093/jnci/58.6.1643
State	Published - Jun 1977
Externally published	Yes

ASJC Scopus subject areas

Oncology
Cancer Research

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title = "Vitamin a contents of rat intestinal epithelium and jejunal mucinous adenocarcinoma",

abstract = "Concentrations of retinol and retinyl esters were assayed in rat intestinal mucosa and in chemically induced transplanted mucinous adenocarcinoma of the jejunum. Lipid extract from the tissues was chromatographed on deactivated alumina to isolate retinol and retinyl esters, which were determined by specific spectrofluorometry. Normal intestinal mucosa tissue contains 556 ng of retinol equivalents as retinyl esters and 303 ng of free retinol/g of wet tissue. The concentration of retinyl esters in the intestinal mucosa from rats carrying the transplanted tumor was 341 ng/g wet tissue; no free retinol was detected in the small intestinal epithelium of these rats. Liver tissue from the tumorbearing rats contained 157 μg of retinol equivalents as retinyl esters and 136 μg of free retinol/g of wet tissue. The concentration of vitamin A per cell in the adenocarcinoma tissue was about 20 times less than that in intestinal epithelium.",

author = "Sundaresan, {P. Ramnathan} and {De Luca}, {Luigi M.}",

note = "Funding Information: 1 Received June 30, 1976; accepted December 7, 1976. 2 Presented in part at the Annual Meeting of the Federation of American Societies for Experimental Biology and Medicine, April 1974 (Fed Proc 33:670, 1974). 3 Supported in part by Public Health Service grant AM13557 from the National Institute of Arthritis, Metabolism, and Digestive Diseases and by Hoffman-La Roche, Inc. 4 Lipids Laboratory Research Institute, St. Joseph Hospital, Lancaster, Pa. 17604. 5Experimental Pathology Branch, National Cancer Institute, National Institutes of Health, Public Health Service, U.S. Department of Health, Education, and Welfare, Bethesda, Md. 20014. 6 Address reprint requests to Dr. De Luca. 7 We thank Ms. Carolyn Wood for her expert technical assistance and Dr. Jerrold Ward for suggestions and discussion of the data.",

year = "1977",

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doi = "10.1093/jnci/58.6.1643",

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AU - Sundaresan, P. Ramnathan

AU - De Luca, Luigi M.

N1 - Funding Information: 1 Received June 30, 1976; accepted December 7, 1976. 2 Presented in part at the Annual Meeting of the Federation of American Societies for Experimental Biology and Medicine, April 1974 (Fed Proc 33:670, 1974). 3 Supported in part by Public Health Service grant AM13557 from the National Institute of Arthritis, Metabolism, and Digestive Diseases and by Hoffman-La Roche, Inc. 4 Lipids Laboratory Research Institute, St. Joseph Hospital, Lancaster, Pa. 17604. 5Experimental Pathology Branch, National Cancer Institute, National Institutes of Health, Public Health Service, U.S. Department of Health, Education, and Welfare, Bethesda, Md. 20014. 6 Address reprint requests to Dr. De Luca. 7 We thank Ms. Carolyn Wood for her expert technical assistance and Dr. Jerrold Ward for suggestions and discussion of the data.

PY - 1977/6

Y1 - 1977/6

N2 - Concentrations of retinol and retinyl esters were assayed in rat intestinal mucosa and in chemically induced transplanted mucinous adenocarcinoma of the jejunum. Lipid extract from the tissues was chromatographed on deactivated alumina to isolate retinol and retinyl esters, which were determined by specific spectrofluorometry. Normal intestinal mucosa tissue contains 556 ng of retinol equivalents as retinyl esters and 303 ng of free retinol/g of wet tissue. The concentration of retinyl esters in the intestinal mucosa from rats carrying the transplanted tumor was 341 ng/g wet tissue; no free retinol was detected in the small intestinal epithelium of these rats. Liver tissue from the tumorbearing rats contained 157 μg of retinol equivalents as retinyl esters and 136 μg of free retinol/g of wet tissue. The concentration of vitamin A per cell in the adenocarcinoma tissue was about 20 times less than that in intestinal epithelium.

AB - Concentrations of retinol and retinyl esters were assayed in rat intestinal mucosa and in chemically induced transplanted mucinous adenocarcinoma of the jejunum. Lipid extract from the tissues was chromatographed on deactivated alumina to isolate retinol and retinyl esters, which were determined by specific spectrofluorometry. Normal intestinal mucosa tissue contains 556 ng of retinol equivalents as retinyl esters and 303 ng of free retinol/g of wet tissue. The concentration of retinyl esters in the intestinal mucosa from rats carrying the transplanted tumor was 341 ng/g wet tissue; no free retinol was detected in the small intestinal epithelium of these rats. Liver tissue from the tumorbearing rats contained 157 μg of retinol equivalents as retinyl esters and 136 μg of free retinol/g of wet tissue. The concentration of vitamin A per cell in the adenocarcinoma tissue was about 20 times less than that in intestinal epithelium.

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Vitamin a contents of rat intestinal epithelium and jejunal mucinous adenocarcinoma

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