Verification and unmasking of widely used human esophageal adenocarcinoma cell lines

Jurjen J. Boonstra; Ronald Van Marion; David G. Beer; Lin Lin; Paula Chaves; Catarina Ribeiro; A. Dias Pereira; Lúcia Roque; S. Jane Darnton; Nasser K. Altorki; David S. Schrump; David S. Klimstra; Laura H. Tang; James R. Eshleman; Hector Alvarez; Yutaka Shimada; Herman Van Dekken; Hugo W. Tilanus; Winand N.M. Dinjens

doi:10.1093/jnci/djp499

Verification and unmasking of widely used human esophageal adenocarcinoma cell lines

Jurjen J. Boonstra, Ronald Van Marion, David G. Beer, Lin Lin, Paula Chaves, Catarina Ribeiro, A. Dias Pereira, Lúcia Roque, S. Jane Darnton, Nasser K. Altorki, David S. Schrump, David S. Klimstra, Laura H. Tang, James R. Eshleman, Hector Alvarez, Yutaka Shimada, Herman Van Dekken, Hugo W. Tilanus, Winand N.M. Dinjens

School of Medicine

Research output: Contribution to journal › Article › peer-review

102 Scopus citations

Abstract

For decades, hundreds of different human tumor type-specific cell lines have been used in experimental cancer research as models for their respective tumors. The veracity of experimental results for a specific tumor type relies on the correct derivation of the cell line. In a worldwide effort, we verified the authenticity of all available esophageal adenocarcinoma (EAC) cell lines. We proved that the frequently used cell lines SEG-1 and BIC-1 and the SK-GT-5 cell line are in fact cell lines from other tumor types. Experimental results based on these contaminated cell lines have led to ongoing clinical trials recruiting EAC patients, to more than 100 scientific publications, and to at least three National Institutes of Health cancer research grants and 11 US patents, which emphasizes the importance of our findings. Widespread use of contaminated cell lines threatens the development of treatment strategies for EAC.

Original language	English (US)
Pages (from-to)	271-274
Number of pages	4
Journal	Journal of the National Cancer Institute
Volume	102
Issue number	4
DOIs	https://doi.org/10.1093/jnci/djp499
State	Published - Feb 2010

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1093/jnci/djp499

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Boonstra, J. J., Van Marion, R., Beer, D. G., Lin, L., Chaves, P., Ribeiro, C., Pereira, A. D., Roque, L., Darnton, S. J., Altorki, N. K., Schrump, D. S., Klimstra, D. S., Tang, L. H., Eshleman, J. R., Alvarez, H., Shimada, Y., Van Dekken, H., Tilanus, H. W., & Dinjens, W. N. M. (2010). Verification and unmasking of widely used human esophageal adenocarcinoma cell lines. Journal of the National Cancer Institute, 102(4), 271-274. https://doi.org/10.1093/jnci/djp499

Boonstra, JJ, Van Marion, R, Beer, DG, Lin, L, Chaves, P, Ribeiro, C, Pereira, AD, Roque, L, Darnton, SJ, Altorki, NK, Schrump, DS, Klimstra, DS, Tang, LH, Eshleman, JR, Alvarez, H, Shimada, Y, Van Dekken, H, Tilanus, HW & Dinjens, WNM 2010, 'Verification and unmasking of widely used human esophageal adenocarcinoma cell lines', Journal of the National Cancer Institute, vol. 102, no. 4, pp. 271-274. https://doi.org/10.1093/jnci/djp499

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abstract = "For decades, hundreds of different human tumor type-specific cell lines have been used in experimental cancer research as models for their respective tumors. The veracity of experimental results for a specific tumor type relies on the correct derivation of the cell line. In a worldwide effort, we verified the authenticity of all available esophageal adenocarcinoma (EAC) cell lines. We proved that the frequently used cell lines SEG-1 and BIC-1 and the SK-GT-5 cell line are in fact cell lines from other tumor types. Experimental results based on these contaminated cell lines have led to ongoing clinical trials recruiting EAC patients, to more than 100 scientific publications, and to at least three National Institutes of Health cancer research grants and 11 US patents, which emphasizes the importance of our findings. Widespread use of contaminated cell lines threatens the development of treatment strategies for EAC.",

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Verification and unmasking of widely used human esophageal adenocarcinoma cell lines

Abstract

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