Vascular Smooth Muscle Actions and Receptor Interactions of 8-Iso-prostaglandin E2, an E2-Isoprostane

M. Fukunaga; K. Takahashi; K. F. Badr

doi:10.1006/bbrc.1993.2075

Vascular Smooth Muscle Actions and Receptor Interactions of 8-Iso-prostaglandin E2, an E2-Isoprostane

M. Fukunaga, K. Takahashi, K. F. Badr

Research output: Contribution to journal › Article › peer-review

74 Scopus citations

Abstract

8-iso-PGE₂, an E2-isoprostane, decreased GFR and RPF dose-dependently in rats, but with lesser potency than 8-epi-PGF_2α, an F2-isoprostane. This effect was abolished by SQ29,548. 8-iso-PGE₂ displaced [³H]SQ29,548 or [¹²⁵I]BOP binding in aortic smooth muscle cells with the affinity rank order of SQ29,548 > = I-BOP > U46,619 > 8-iso-PGE₂ > PGF_2α, while it activated phospholipase C with a potency greater than those of I-BOP or U46,619 and lesser than that of 8-epi-PGF_2α. We concluded that 8-iso-PGE₂ is a renal vasoconstrictor linked to phosphoinositide metabolism. Its vascular smooth muscle contractile actions are likely mediated through activation of putative thromboxane A₂ receptor-like "isoprostane receptors".

Original language	English (US)
Pages (from-to)	507-515
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	195
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1993.2075
State	Published - Sep 15 1993
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry

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title = "Vascular Smooth Muscle Actions and Receptor Interactions of 8-Iso-prostaglandin E2, an E2-Isoprostane",

abstract = "8-iso-PGE2, an E2-isoprostane, decreased GFR and RPF dose-dependently in rats, but with lesser potency than 8-epi-PGF2α, an F2-isoprostane. This effect was abolished by SQ29,548. 8-iso-PGE2 displaced [3H]SQ29,548 or [125I]BOP binding in aortic smooth muscle cells with the affinity rank order of SQ29,548 > = I-BOP > U46,619 > 8-iso-PGE2 > PGF2α, while it activated phospholipase C with a potency greater than those of I-BOP or U46,619 and lesser than that of 8-epi-PGF2α. We concluded that 8-iso-PGE2 is a renal vasoconstrictor linked to phosphoinositide metabolism. Its vascular smooth muscle contractile actions are likely mediated through activation of putative thromboxane A2 receptor-like {"}isoprostane receptors{"}.",

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N2 - 8-iso-PGE2, an E2-isoprostane, decreased GFR and RPF dose-dependently in rats, but with lesser potency than 8-epi-PGF2α, an F2-isoprostane. This effect was abolished by SQ29,548. 8-iso-PGE2 displaced [3H]SQ29,548 or [125I]BOP binding in aortic smooth muscle cells with the affinity rank order of SQ29,548 > = I-BOP > U46,619 > 8-iso-PGE2 > PGF2α, while it activated phospholipase C with a potency greater than those of I-BOP or U46,619 and lesser than that of 8-epi-PGF2α. We concluded that 8-iso-PGE2 is a renal vasoconstrictor linked to phosphoinositide metabolism. Its vascular smooth muscle contractile actions are likely mediated through activation of putative thromboxane A2 receptor-like "isoprostane receptors".

AB - 8-iso-PGE2, an E2-isoprostane, decreased GFR and RPF dose-dependently in rats, but with lesser potency than 8-epi-PGF2α, an F2-isoprostane. This effect was abolished by SQ29,548. 8-iso-PGE2 displaced [3H]SQ29,548 or [125I]BOP binding in aortic smooth muscle cells with the affinity rank order of SQ29,548 > = I-BOP > U46,619 > 8-iso-PGE2 > PGF2α, while it activated phospholipase C with a potency greater than those of I-BOP or U46,619 and lesser than that of 8-epi-PGF2α. We concluded that 8-iso-PGE2 is a renal vasoconstrictor linked to phosphoinositide metabolism. Its vascular smooth muscle contractile actions are likely mediated through activation of putative thromboxane A2 receptor-like "isoprostane receptors".

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Vascular Smooth Muscle Actions and Receptor Interactions of 8-Iso-prostaglandin E2, an E2-Isoprostane

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