Abstract
The oncogene N-ras has been found to be amplified (20 copies) in the human breast carcinoma cell line MCF-7. The amplified sequences have been localized to a marker chromosome by in situ hybridization. Sublines of MCF-7, serially passaged in different laboratories; have marked variation in the degree of N-ras amplification. The differing degrees of amplification of N-ras are further evidence of heterogeneity within MCF-7 subclones. The phenomenon may not have general relevance for breast cancer, since other breast cancer cell lines and DNA from patient biopsies failed to show evidence of N-ras amplification.
Original language | English (US) |
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Pages (from-to) | 2201-2205 |
Number of pages | 5 |
Journal | Cancer Research |
Volume | 45 |
Issue number | 5 |
State | Published - May 1 1985 |
Externally published | Yes |
ASJC Scopus subject areas
- Oncology
- Cancer Research