@article{fe9ab081d6354a49be4155371f1a5a9f,
title = "Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates",
abstract = "Micali et al. report that human telencephalic NSCs in vitro transition through the organizer states that pattern the neocortex. Human pluripotent lines vary in organizer formation, generating divergent neuronal differentiation trajectories biased toward dorsal or ventral telencephalic fates and opening further analysis of the earliest cortical specification events.",
keywords = "BMP, EGFR, FGF2, brain organizer, glutamatergic neurons, human iPSC variation, inhibitory neurons, neural stem cell, neural transcriptional dynamics, neurogenesis, neuronal trajectory variation, patterning of the cortex",
author = "Nicola Micali and Kim, {Suel Kee} and Marcelo Diaz-Bustamante and Genevieve Stein-O'Brien and Seungmae Seo and Shin, {Joo Heon} and Rash, {Brian G.} and Shaojie Ma and Yanhong Wang and Olivares, {Nicolas A.} and Arellano, {Jon I.} and Maynard, {Kristen R.} and Fertig, {Elana J.} and Cross, {Alan J.} and B{\"u}rli, {Roland W.} and Brandon, {Nicholas J.} and Weinberger, {Daniel R.} and Chenoweth, {Joshua G.} and Hoeppner, {Daniel J.} and Nenad Sestan and Pasko Rakic and Carlo Colantuoni and McKay, {Ronald D.}",
note = "Funding Information: This work was supported by the Lieber Institute for Brain Development (LIBD), AstraZeneca, and NIH grants DA02399 and EY002593 (to P.R.) and MH106934 , MH106874 , MH116488 , MH110926 , and MH109904 (to N.S.). E.J.F. and G.S.-O. were supported by the NIH ( CA177669 , CA212007 , and CA006973 ), the Johns Hopkins University Discovery Award , and the Johns Hopkins School of Medicine Synergy Award . We thank many members of LIBD and the Yale University Department of Neuroscience (Rakic and {\v S}estan labs) for their helpful comments on this work and manuscript. We thank Dr. Alvaro Duque (Rakic lab) and MacBrainResource ( https://medicine.yale.edu/neuroscience/macbrain/ ) for providing non-human primate (NHP) tissue ( National Institute of Mental Health (NIMH) R01-MH113257 to Alvaro Duque). Funding Information: This work was supported by the Lieber Institute for Brain Development (LIBD), AstraZeneca, and NIH grants DA02399 and EY002593 (to P.R.) and MH106934, MH106874, MH116488, MH110926, and MH109904 (to N.S.). E.J.F. and G.S.-O. were supported by the NIH (CA177669, CA212007, and CA006973), the Johns Hopkins University Discovery Award, and the Johns Hopkins School of Medicine Synergy Award. We thank many members of LIBD and the Yale University Department of Neuroscience (Rakic and ?estan labs) for their helpful comments on this work and manuscript. We thank Dr. Alvaro Duque (Rakic lab) and MacBrainResource (https://medicine.yale.edu/neuroscience/macbrain/) for providing non-human primate (NHP) tissue (National Institute of Mental Health (NIMH) R01-MH113257 to Alvaro Duque). N.M. and R.D.M. conceived the study. N.M. performed the experiments and data collection. N.M. and S.-K.K. performed the stem cell culture. M.D.-B. performed the e-physiology. Y.W. generated the hiPSC lines. J.-H.S. generated the bulk RNA-seq data. G.S.-O. E.J.F. J.-H.S. and C.C. processed and analyzed the bulk RNA-seq data. S.M. and C.C. analyzed the scRNA-seq data. N.M. S.-K.K. S.S. and D.J.H. performed the immunofluorescence (IF), imaging, and high-content image analysis. N.M. C.C. G.S.-O. and D.J.H. performed the lineage analysis. N.M. and K.R.M. performed the neuromorphology analysis. B.G.R. performed the immunohistochemistry (IHC) and imaging of the monkey tissue slides. J.I.A. banked the monkey tissue. N.M. S.-K.K. G.S.-O. S.S. M.D.-B. N.A.O. J.G.C. C.C. and D.J.H. developed the biological and bio-informatic pipelines to manipulate and analyze the hPSCs. N.M. S.-K.K. G.S.-O. D.J.H. C.C. N.S. P.R. and R.D.M. interpreted the data. A.J.C. R.W.B. N.J.B. D.R.W. N.S. P.R. and R.D.M. directed the research. N.M. S.-K.K. P.R. C.C. and R.D.M. wrote the manuscript. All of the authors participated in discussions of the results and manuscript editing. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2020 The Authors",
year = "2020",
month = may,
day = "5",
doi = "10.1016/j.celrep.2020.107599",
language = "English (US)",
volume = "31",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "5",
}