Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates

Nicola Micali; Suel Kee Kim; Marcelo Diaz-Bustamante; Genevieve Stein-O'Brien; Seungmae Seo; Joo Heon Shin; Brian G. Rash; Shaojie Ma; Yanhong Wang; Nicolas A. Olivares; Jon I. Arellano; Kristen R. Maynard; Elana J. Fertig; Alan J. Cross; Roland W. Bürli; Nicholas J. Brandon; Daniel R. Weinberger; Joshua G. Chenoweth; Daniel J. Hoeppner; Nenad Sestan; Pasko Rakic; Carlo Colantuoni; Ronald D. McKay

doi:10.1016/j.celrep.2020.107599

Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates

Nicola Micali, Suel Kee Kim, Marcelo Diaz-Bustamante, Genevieve Stein-O'Brien, Seungmae Seo, Joo Heon Shin, Brian G. Rash, Shaojie Ma, Yanhong Wang, Nicolas A. Olivares, Jon I. Arellano, Kristen R. Maynard, Elana J. Fertig, Alan J. Cross, Roland W. Bürli, Nicholas J. Brandon, Daniel R. Weinberger, Joshua G. Chenoweth, Daniel J. Hoeppner, Nenad SestanPasko Rakic, Carlo Colantuoni, Ronald D. McKay

School of Medicine

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Micali et al. report that human telencephalic NSCs in vitro transition through the organizer states that pattern the neocortex. Human pluripotent lines vary in organizer formation, generating divergent neuronal differentiation trajectories biased toward dorsal or ventral telencephalic fates and opening further analysis of the earliest cortical specification events.

Original language	English (US)
Article number	107599
Journal	Cell Reports
Volume	31
Issue number	5
DOIs	https://doi.org/10.1016/j.celrep.2020.107599
State	Published - May 5 2020

Keywords

BMP
EGFR
FGF2
brain organizer
glutamatergic neurons
human iPSC variation
inhibitory neurons
neural stem cell
neural transcriptional dynamics
neurogenesis
neuronal trajectory variation
patterning of the cortex

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/j.celrep.2020.107599

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Micali, N., Kim, S. K., Diaz-Bustamante, M., Stein-O'Brien, G., Seo, S., Shin, J. H., Rash, B. G., Ma, S., Wang, Y., Olivares, N. A., Arellano, J. I., Maynard, K. R., Fertig, E. J., Cross, A. J., Bürli, R. W., Brandon, N. J., Weinberger, D. R., Chenoweth, J. G., Hoeppner, D. J., ... McKay, R. D. (2020). Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates. Cell Reports, 31(5), Article 107599. https://doi.org/10.1016/j.celrep.2020.107599

Micali, N, Kim, SK, Diaz-Bustamante, M, Stein-O'Brien, G, Seo, S, Shin, JH, Rash, BG, Ma, S, Wang, Y, Olivares, NA, Arellano, JI, Maynard, KR, Fertig, EJ, Cross, AJ, Bürli, RW, Brandon, NJ, Weinberger, DR, Chenoweth, JG, Hoeppner, DJ, Sestan, N, Rakic, P, Colantuoni, C & McKay, RD 2020, 'Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates', Cell Reports, vol. 31, no. 5, 107599. https://doi.org/10.1016/j.celrep.2020.107599

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title = "Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates",

abstract = "Micali et al. report that human telencephalic NSCs in vitro transition through the organizer states that pattern the neocortex. Human pluripotent lines vary in organizer formation, generating divergent neuronal differentiation trajectories biased toward dorsal or ventral telencephalic fates and opening further analysis of the earliest cortical specification events.",

keywords = "BMP, EGFR, FGF2, brain organizer, glutamatergic neurons, human iPSC variation, inhibitory neurons, neural stem cell, neural transcriptional dynamics, neurogenesis, neuronal trajectory variation, patterning of the cortex",

author = "Nicola Micali and Kim, {Suel Kee} and Marcelo Diaz-Bustamante and Genevieve Stein-O'Brien and Seungmae Seo and Shin, {Joo Heon} and Rash, {Brian G.} and Shaojie Ma and Yanhong Wang and Olivares, {Nicolas A.} and Arellano, {Jon I.} and Maynard, {Kristen R.} and Fertig, {Elana J.} and Cross, {Alan J.} and B{\"u}rli, {Roland W.} and Brandon, {Nicholas J.} and Weinberger, {Daniel R.} and Chenoweth, {Joshua G.} and Hoeppner, {Daniel J.} and Nenad Sestan and Pasko Rakic and Carlo Colantuoni and McKay, {Ronald D.}",

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AU - Kim, Suel Kee

AU - Diaz-Bustamante, Marcelo

AU - Stein-O'Brien, Genevieve

AU - Seo, Seungmae

AU - Shin, Joo Heon

AU - Rash, Brian G.

AU - Ma, Shaojie

AU - Wang, Yanhong

AU - Olivares, Nicolas A.

AU - Arellano, Jon I.

AU - Maynard, Kristen R.

AU - Fertig, Elana J.

AU - Cross, Alan J.

AU - Bürli, Roland W.

AU - Brandon, Nicholas J.

AU - Weinberger, Daniel R.

AU - Chenoweth, Joshua G.

AU - Hoeppner, Daniel J.

AU - Sestan, Nenad

AU - Rakic, Pasko

AU - Colantuoni, Carlo

AU - McKay, Ronald D.

PY - 2020/5/5

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AB - Micali et al. report that human telencephalic NSCs in vitro transition through the organizer states that pattern the neocortex. Human pluripotent lines vary in organizer formation, generating divergent neuronal differentiation trajectories biased toward dorsal or ventral telencephalic fates and opening further analysis of the earliest cortical specification events.

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KW - EGFR

KW - FGF2

KW - brain organizer

KW - glutamatergic neurons

KW - human iPSC variation

KW - inhibitory neurons

KW - neural stem cell

KW - neural transcriptional dynamics

KW - neurogenesis

KW - neuronal trajectory variation

KW - patterning of the cortex

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Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates

Abstract

Keywords

ASJC Scopus subject areas

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