Variation at the capsule locus, cps, of mistyped and non-typable Streptococcus pneumoniae isolates

S. J. Salter; J. Hinds; K. A. Gould; L. Lambertsen; W. Hanage; M. Antonio; P. Turner; P. W.M. Hermans; H. J. Bootsma; K. L. O'Brien; S. D. Bentley

doi:10.1099/mic.0.056580-0

Variation at the capsule locus, cps, of mistyped and non-typable Streptococcus pneumoniae isolates

S. J. Salter, J. Hinds, K. A. Gould, L. Lambertsen, W. Hanage, M. Antonio, P. Turner, P. W.M. Hermans, H. J. Bootsma, K. L. O'Brien, S. D. Bentley

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

The capsule polysaccharide locus (cps) is the site of the capsule biosynthesis gene cluster in encapsulated Streptococcus pneumoniae. A set of pneumococcal samples and nonpneumococcal streptococci from Denmark, the Gambia, the Netherlands, Thailand, the UK and the USA were sequenced at the cps locus to elucidate serologically mistyped or non-typable isolates. We identified a novel serotype 33B/33C mosaic capsule cluster and previously unseen serotype 22F capsule genes, disrupted and deleted cps clusters, the presence of aliB and nspA genes that are unrelated to capsule production, and similar genes in the non-pneumococcal samples. These data provide greater understanding of diversity at a locus which is crucial to the antigenic diversity of the pathogen and current vaccine strategies.

Original language	English (US)
Pages (from-to)	1560-1569
Number of pages	10
Journal	Microbiology (United Kingdom)
Volume	158
Issue number	6
DOIs	https://doi.org/10.1099/mic.0.056580-0
State	Published - Jun 2012
Externally published	Yes

ASJC Scopus subject areas

Microbiology

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title = "Variation at the capsule locus, cps, of mistyped and non-typable Streptococcus pneumoniae isolates",

abstract = "The capsule polysaccharide locus (cps) is the site of the capsule biosynthesis gene cluster in encapsulated Streptococcus pneumoniae. A set of pneumococcal samples and nonpneumococcal streptococci from Denmark, the Gambia, the Netherlands, Thailand, the UK and the USA were sequenced at the cps locus to elucidate serologically mistyped or non-typable isolates. We identified a novel serotype 33B/33C mosaic capsule cluster and previously unseen serotype 22F capsule genes, disrupted and deleted cps clusters, the presence of aliB and nspA genes that are unrelated to capsule production, and similar genes in the non-pneumococcal samples. These data provide greater understanding of diversity at a locus which is crucial to the antigenic diversity of the pathogen and current vaccine strategies.",

author = "Salter, {S. J.} and J. Hinds and Gould, {K. A.} and L. Lambertsen and W. Hanage and M. Antonio and P. Turner and Hermans, {P. W.M.} and Bootsma, {H. J.} and O'Brien, {K. L.} and Bentley, {S. D.}",

year = "2012",

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doi = "10.1099/mic.0.056580-0",

language = "English (US)",

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AU - Salter, S. J.

AU - Hinds, J.

AU - Gould, K. A.

AU - Lambertsen, L.

AU - Hanage, W.

AU - Antonio, M.

AU - Turner, P.

AU - Hermans, P. W.M.

AU - Bootsma, H. J.

AU - O'Brien, K. L.

AU - Bentley, S. D.

PY - 2012/6

Y1 - 2012/6

N2 - The capsule polysaccharide locus (cps) is the site of the capsule biosynthesis gene cluster in encapsulated Streptococcus pneumoniae. A set of pneumococcal samples and nonpneumococcal streptococci from Denmark, the Gambia, the Netherlands, Thailand, the UK and the USA were sequenced at the cps locus to elucidate serologically mistyped or non-typable isolates. We identified a novel serotype 33B/33C mosaic capsule cluster and previously unseen serotype 22F capsule genes, disrupted and deleted cps clusters, the presence of aliB and nspA genes that are unrelated to capsule production, and similar genes in the non-pneumococcal samples. These data provide greater understanding of diversity at a locus which is crucial to the antigenic diversity of the pathogen and current vaccine strategies.

AB - The capsule polysaccharide locus (cps) is the site of the capsule biosynthesis gene cluster in encapsulated Streptococcus pneumoniae. A set of pneumococcal samples and nonpneumococcal streptococci from Denmark, the Gambia, the Netherlands, Thailand, the UK and the USA were sequenced at the cps locus to elucidate serologically mistyped or non-typable isolates. We identified a novel serotype 33B/33C mosaic capsule cluster and previously unseen serotype 22F capsule genes, disrupted and deleted cps clusters, the presence of aliB and nspA genes that are unrelated to capsule production, and similar genes in the non-pneumococcal samples. These data provide greater understanding of diversity at a locus which is crucial to the antigenic diversity of the pathogen and current vaccine strategies.

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Variation at the capsule locus, cps, of mistyped and non-typable Streptococcus pneumoniae isolates

Abstract

ASJC Scopus subject areas

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