Variability in beta-globin and HPV DNA amplification by PCR from fixed tissues.

J. S. Park, J. F. Leake, B. K. Sharma, T. Toki, T. D. Kessis, R. A. Ambros, K. V. Shah

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18 Scopus citations


Paraffin-embedded tissues from a variety of sources and treated with different fixatives were tested for beta-globin and HPV amplification by polymerase chain reaction (PCR). In tests of tissues collected in the previous 2 to 3 yr, excellent rates (87% to 93%) for beta-globin amplification were obtained for specimens fixed in buffered formalin, Bouin's, and Hartmann's solutions. In contrast, the rate of beta-globin amplification was low for tissues fixed in Hollande's solution (7%) and in Hartmann's solution with eosin (33%). The results of beta-globin amplification from archival tissues stored for variable time periods showed no decrease in the amplification rate with longer periods of storage. Human papillomaviruses (HPVs) were identified in 17% of globin negative and in 43% of globin positive tissues. HPV-16 amplification was more efficient when the targeted DNA sequence was small. Variability in amplification depends not only on the type of fixative used, but also on other ill-defined factors. Therefore, conditions for optimal amplification should be determined before undertaking studies of archival material.

Original languageEnglish (US)
Pages (from-to)667-670
Number of pages4
JournalModern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
Issue number5
StatePublished - 1991
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)


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