Vanadate inhibition of hyaluronic acid synthesis in Swiss 3T3 fibroblasts

D. W. Zaharevitz; C. A. Chisena; K. L.K. Duncan; E. M. August; R. L. Cysyk

Vanadate inhibition of hyaluronic acid synthesis in Swiss 3T3 fibroblasts

D. W. Zaharevitz, C. A. Chisena, K. L.K. Duncan, E. M. August, R. L. Cysyk

School of Medicine

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Vanadate (50 μM) increases the incorporation of ³H from D-[1,6^-3H]glucosamine into hyaluronic acid (HA) in serum stimulated Swiss 3T3 fibroblasts. This increase is not a result of increased HA synthesis, but is due to a significant increase in the specific activity of the cellular UDP-N-acetyl-glucosamine. When the ³H incorporation is corrected for the change in precursor specific activity, vanadate is shown to inhibit the synthesis of HA, a result that is confirmed by measuring the HA with a competitive binding assay. The inhibition of HA synthesis by vanadate is also shown to be dependent on the media used in the experiment, with a substantial increase in the inhibition seen when the media contains the pH indicator, phenol red. The HA synthetase activity of isolated membranes is not inhibited by vanadate at concentrations up to 500 μM.

Original language	English (US)
Pages (from-to)	627-633
Number of pages	7
Journal	Biochemistry and Molecular Biology International
Volume	31
Issue number	4
State	Published - Jan 1 1993

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Genetics

Cite this

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title = "Vanadate inhibition of hyaluronic acid synthesis in Swiss 3T3 fibroblasts",

abstract = "Vanadate (50 μM) increases the incorporation of 3H from D-[1,6-3H]glucosamine into hyaluronic acid (HA) in serum stimulated Swiss 3T3 fibroblasts. This increase is not a result of increased HA synthesis, but is due to a significant increase in the specific activity of the cellular UDP-N-acetyl-glucosamine. When the 3H incorporation is corrected for the change in precursor specific activity, vanadate is shown to inhibit the synthesis of HA, a result that is confirmed by measuring the HA with a competitive binding assay. The inhibition of HA synthesis by vanadate is also shown to be dependent on the media used in the experiment, with a substantial increase in the inhibition seen when the media contains the pH indicator, phenol red. The HA synthetase activity of isolated membranes is not inhibited by vanadate at concentrations up to 500 μM.",

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T1 - Vanadate inhibition of hyaluronic acid synthesis in Swiss 3T3 fibroblasts

AU - Zaharevitz, D. W.

AU - Chisena, C. A.

AU - Duncan, K. L.K.

AU - August, E. M.

AU - Cysyk, R. L.

PY - 1993/1/1

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N2 - Vanadate (50 μM) increases the incorporation of 3H from D-[1,6-3H]glucosamine into hyaluronic acid (HA) in serum stimulated Swiss 3T3 fibroblasts. This increase is not a result of increased HA synthesis, but is due to a significant increase in the specific activity of the cellular UDP-N-acetyl-glucosamine. When the 3H incorporation is corrected for the change in precursor specific activity, vanadate is shown to inhibit the synthesis of HA, a result that is confirmed by measuring the HA with a competitive binding assay. The inhibition of HA synthesis by vanadate is also shown to be dependent on the media used in the experiment, with a substantial increase in the inhibition seen when the media contains the pH indicator, phenol red. The HA synthetase activity of isolated membranes is not inhibited by vanadate at concentrations up to 500 μM.

AB - Vanadate (50 μM) increases the incorporation of 3H from D-[1,6-3H]glucosamine into hyaluronic acid (HA) in serum stimulated Swiss 3T3 fibroblasts. This increase is not a result of increased HA synthesis, but is due to a significant increase in the specific activity of the cellular UDP-N-acetyl-glucosamine. When the 3H incorporation is corrected for the change in precursor specific activity, vanadate is shown to inhibit the synthesis of HA, a result that is confirmed by measuring the HA with a competitive binding assay. The inhibition of HA synthesis by vanadate is also shown to be dependent on the media used in the experiment, with a substantial increase in the inhibition seen when the media contains the pH indicator, phenol red. The HA synthetase activity of isolated membranes is not inhibited by vanadate at concentrations up to 500 μM.

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Vanadate inhibition of hyaluronic acid synthesis in Swiss 3T3 fibroblasts

Abstract

ASJC Scopus subject areas

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