Validated liquid chromatographic-electrospray ionization mass spectrometric assay for simultaneous determination of 3,4-methylenedioxymethamphetamine and its metabolites 3,4-methylenedioxyamphetamine, 3,4-dihydroxymethamphetamine, and 4-hydroxy-3-methoxymethamphetamine in squirrel monkey plasma

Melanie Mueller; Frank T. Peters; George A. Ricaurte; Hans H. Maurer

doi:10.1016/j.jchromb.2007.06.034

Validated liquid chromatographic-electrospray ionization mass spectrometric assay for simultaneous determination of 3,4-methylenedioxymethamphetamine and its metabolites 3,4-methylenedioxyamphetamine, 3,4-dihydroxymethamphetamine, and 4-hydroxy-3-methoxymethamphetamine in squirrel monkey plasma

Melanie Mueller, Frank T. Peters, George A. Ricaurte, Hans H. Maurer

School of Medicine

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

3,4-Methylenedioxymethamphetamine (MDMA) is a recreational drug with neurotoxic potential. Pharmacokinetic data of MDMA and its metabolites may shed light on the mechanism of MDMA neurotoxicity. An LC-MS assay with electrospray ionization (ESI) is presented for quantifying MDMA and its metabolites 3,4-methylenedioxyamphetamine (MDA), 3,4-dihydroxymethamphetamine (HHMA), and 4-hydroxy-3-methoxymethamphetamine (HMMA) in squirrel monkey plasma. The method involved enzymatic conjugate cleavage and protein precipitation. Separation was achieved within 14 min. The method was validated according to international guidelines with respect to selectivity, linearity, accuracy, precision, recovery, and matrix effect. The present method should prove useful for acquiring pharmacokinetic and toxicokinetic data in squirrel monkeys.

Original language	English (US)
Pages (from-to)	262-270
Number of pages	9
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	855
Issue number	2 SPEC. ISS.
DOIs	https://doi.org/10.1016/j.jchromb.2007.06.034
State	Published - Aug 15 2007

Keywords

LC-ESI-MS
MDMA
Metabolites
Neurotoxicity
Plasma
Validation

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry
Cell Biology

Access to Document

10.1016/j.jchromb.2007.06.034

Fingerprint

Dive into the research topics of 'Validated liquid chromatographic-electrospray ionization mass spectrometric assay for simultaneous determination of 3,4-methylenedioxymethamphetamine and its metabolites 3,4-methylenedioxyamphetamine, 3,4-dihydroxymethamphetamine, and 4-hydroxy-3-methoxymethamphetamine in squirrel monkey plasma'. Together they form a unique fingerprint.

Cite this

Mueller, M., Peters, F. T., Ricaurte, G. A., & Maurer, H. H. (2007). Validated liquid chromatographic-electrospray ionization mass spectrometric assay for simultaneous determination of 3,4-methylenedioxymethamphetamine and its metabolites 3,4-methylenedioxyamphetamine, 3,4-dihydroxymethamphetamine, and 4-hydroxy-3-methoxymethamphetamine in squirrel monkey plasma. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 855(2 SPEC. ISS.), 262-270. https://doi.org/10.1016/j.jchromb.2007.06.034

Validated liquid chromatographic-electrospray ionization mass spectrometric assay for simultaneous determination of 3,4-methylenedioxymethamphetamine and its metabolites 3,4-methylenedioxyamphetamine, 3,4-dihydroxymethamphetamine, and 4-hydroxy-3-methoxymethamphetamine in squirrel monkey plasma. / Mueller, Melanie; Peters, Frank T.; Ricaurte, George A. et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 855, No. 2 SPEC. ISS., 15.08.2007, p. 262-270.

Research output: Contribution to journal › Article › peer-review

Mueller, M, Peters, FT, Ricaurte, GA & Maurer, HH 2007, 'Validated liquid chromatographic-electrospray ionization mass spectrometric assay for simultaneous determination of 3,4-methylenedioxymethamphetamine and its metabolites 3,4-methylenedioxyamphetamine, 3,4-dihydroxymethamphetamine, and 4-hydroxy-3-methoxymethamphetamine in squirrel monkey plasma', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 855, no. 2 SPEC. ISS., pp. 262-270. https://doi.org/10.1016/j.jchromb.2007.06.034

Mueller M, Peters FT, Ricaurte GA, Maurer HH. Validated liquid chromatographic-electrospray ionization mass spectrometric assay for simultaneous determination of 3,4-methylenedioxymethamphetamine and its metabolites 3,4-methylenedioxyamphetamine, 3,4-dihydroxymethamphetamine, and 4-hydroxy-3-methoxymethamphetamine in squirrel monkey plasma. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2007 Aug 15;855(2 SPEC. ISS.):262-270. doi: 10.1016/j.jchromb.2007.06.034

Mueller, Melanie ; Peters, Frank T. ; Ricaurte, George A. et al. / Validated liquid chromatographic-electrospray ionization mass spectrometric assay for simultaneous determination of 3,4-methylenedioxymethamphetamine and its metabolites 3,4-methylenedioxyamphetamine, 3,4-dihydroxymethamphetamine, and 4-hydroxy-3-methoxymethamphetamine in squirrel monkey plasma. In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2007 ; Vol. 855, No. 2 SPEC. ISS. pp. 262-270.

@article{89412404a9634bc59cc5a028b7849249,

title = "Validated liquid chromatographic-electrospray ionization mass spectrometric assay for simultaneous determination of 3,4-methylenedioxymethamphetamine and its metabolites 3,4-methylenedioxyamphetamine, 3,4-dihydroxymethamphetamine, and 4-hydroxy-3-methoxymethamphetamine in squirrel monkey plasma",

abstract = "3,4-Methylenedioxymethamphetamine (MDMA) is a recreational drug with neurotoxic potential. Pharmacokinetic data of MDMA and its metabolites may shed light on the mechanism of MDMA neurotoxicity. An LC-MS assay with electrospray ionization (ESI) is presented for quantifying MDMA and its metabolites 3,4-methylenedioxyamphetamine (MDA), 3,4-dihydroxymethamphetamine (HHMA), and 4-hydroxy-3-methoxymethamphetamine (HMMA) in squirrel monkey plasma. The method involved enzymatic conjugate cleavage and protein precipitation. Separation was achieved within 14 min. The method was validated according to international guidelines with respect to selectivity, linearity, accuracy, precision, recovery, and matrix effect. The present method should prove useful for acquiring pharmacokinetic and toxicokinetic data in squirrel monkeys.",

keywords = "LC-ESI-MS, MDMA, Metabolites, Neurotoxicity, Plasma, Validation",

author = "Melanie Mueller and Peters, {Frank T.} and Ricaurte, {George A.} and Maurer, {Hans H.}",

year = "2007",

month = aug,

day = "15",

doi = "10.1016/j.jchromb.2007.06.034",

language = "English (US)",

volume = "855",

pages = "262--270",

journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

issn = "1570-0232",

publisher = "Elsevier",

number = "2 SPEC. ISS.",

}

TY - JOUR

T1 - Validated liquid chromatographic-electrospray ionization mass spectrometric assay for simultaneous determination of 3,4-methylenedioxymethamphetamine and its metabolites 3,4-methylenedioxyamphetamine, 3,4-dihydroxymethamphetamine, and 4-hydroxy-3-methoxymethamphetamine in squirrel monkey plasma

AU - Mueller, Melanie

AU - Peters, Frank T.

AU - Ricaurte, George A.

AU - Maurer, Hans H.

PY - 2007/8/15

Y1 - 2007/8/15

N2 - 3,4-Methylenedioxymethamphetamine (MDMA) is a recreational drug with neurotoxic potential. Pharmacokinetic data of MDMA and its metabolites may shed light on the mechanism of MDMA neurotoxicity. An LC-MS assay with electrospray ionization (ESI) is presented for quantifying MDMA and its metabolites 3,4-methylenedioxyamphetamine (MDA), 3,4-dihydroxymethamphetamine (HHMA), and 4-hydroxy-3-methoxymethamphetamine (HMMA) in squirrel monkey plasma. The method involved enzymatic conjugate cleavage and protein precipitation. Separation was achieved within 14 min. The method was validated according to international guidelines with respect to selectivity, linearity, accuracy, precision, recovery, and matrix effect. The present method should prove useful for acquiring pharmacokinetic and toxicokinetic data in squirrel monkeys.

AB - 3,4-Methylenedioxymethamphetamine (MDMA) is a recreational drug with neurotoxic potential. Pharmacokinetic data of MDMA and its metabolites may shed light on the mechanism of MDMA neurotoxicity. An LC-MS assay with electrospray ionization (ESI) is presented for quantifying MDMA and its metabolites 3,4-methylenedioxyamphetamine (MDA), 3,4-dihydroxymethamphetamine (HHMA), and 4-hydroxy-3-methoxymethamphetamine (HMMA) in squirrel monkey plasma. The method involved enzymatic conjugate cleavage and protein precipitation. Separation was achieved within 14 min. The method was validated according to international guidelines with respect to selectivity, linearity, accuracy, precision, recovery, and matrix effect. The present method should prove useful for acquiring pharmacokinetic and toxicokinetic data in squirrel monkeys.

KW - LC-ESI-MS

KW - MDMA

KW - Metabolites

KW - Neurotoxicity

KW - Plasma

KW - Validation

UR - http://www.scopus.com/inward/record.url?scp=34547697195&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547697195&partnerID=8YFLogxK

U2 - 10.1016/j.jchromb.2007.06.034

DO - 10.1016/j.jchromb.2007.06.034

M3 - Article

C2 - 17646137

AN - SCOPUS:34547697195

SN - 1570-0232

VL - 855

SP - 262

EP - 270

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 2 SPEC. ISS.

ER -

Validated liquid chromatographic-electrospray ionization mass spectrometric assay for simultaneous determination of 3,4-methylenedioxymethamphetamine and its metabolites 3,4-methylenedioxyamphetamine, 3,4-dihydroxymethamphetamine, and 4-hydroxy-3-methoxymethamphetamine in squirrel monkey plasma

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this