Vagotomy reverses established allergen-induced airway hyperreactivity to methacholine in the mouse

M. Allen McAlexander, Stephen H. Gavett, Marian Kollarik, Bradley J. Undem

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


We evaluated the role of vagal reflexes in a mouse model of allergen-induced airway hyperreactivity. Mice were actively sensitized to ovalbumin then exposed to the allergen via inhalation. Prior to ovalbumin inhalation, mice also received intratracheally-instilled particulate matter in order to boost the allergic response. In control mice, methacholine (i.v.) caused a dose-dependent increase in respiratory tract resistance (RT) that only modestly decreased if the vagi were severed bilaterally just prior to the methacholine challenge. Sensitized and challenged mice, however, manifested an airway reactivity increase that was abolished by severing the vagi prior to methacholine challenge. In an innervated ex vivo mouse lung model, methacholine selectively evoked action potential discharge in a subset of distension-sensitive A-fibers. These data support the hypothesis that the major component of the increased airway reactivity in inflamed mice is due to a vagal reflex initiated by activation of afferent fibers, even in response to a direct (i.e., smooth muscle)-acting muscarinic agonist.

Original languageEnglish (US)
Pages (from-to)20-24
Number of pages5
JournalRespiratory Physiology and Neurobiology
StatePublished - Jul 1 2015


  • Airway hyperreactivity
  • Allergic inflammation
  • Particulate matter
  • Reflex
  • Vagotomy
  • Vagus nerve

ASJC Scopus subject areas

  • General Neuroscience
  • Physiology
  • Pulmonary and Respiratory Medicine


Dive into the research topics of 'Vagotomy reverses established allergen-induced airway hyperreactivity to methacholine in the mouse'. Together they form a unique fingerprint.

Cite this