Utilizing DUET Imaging for Quantification of Collagen and Elastin as Biomarkers for Studying Disease

Willy Ju, Samuel Border, Anupam Mitra, Eniola Okon, Hyunjae Jeong, Richard M. Levenson, Avi Rosenberg, Kuang Yu Jen, Pinaki Sarder, Farzad Fereidouni

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Collagen and elastin are prominent components in both normal and abnormal tissues, and their presence and distribution have great significance for fibrosis- and cancer-related processes. Collagen and elastin quantification in the context of fibrosis, often associated with irreparable organ injury, can predict the disease severity and patient prognosis. In the context of cancer, specific spatial collagen signatures are known to influence tumor microenvironments while identification of elastin is important in the context of treatment of metastatic cancers. Traditional methods to quantify collagen and elastin vary in accuracy, cost, and ease of use. Using DUET microscopy on H&E slides, high-resolution collagen and elastin mapping is possible without added staining steps or expensive optical instrumentation. We demonstrate this approach in chronic kidney disease (CKD), coronary artery disease (CAD), and for identifying vascular elastin in colon cancers.

Original languageEnglish (US)
Title of host publicationReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XV
EditorsMikhail Y. Berezin, Ramesh Raghavachari
PublisherSPIE
ISBN (Electronic)9781510669833
DOIs
StatePublished - 2024
EventReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XV 2024 - San Francisco, United States
Duration: Jan 30 2024Jan 31 2024

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume12862
ISSN (Print)1605-7422

Conference

ConferenceReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XV 2024
Country/TerritoryUnited States
CitySan Francisco
Period1/30/241/31/24

Keywords

  • Collagen
  • Deep Learning
  • Digital Pathology
  • EVG
  • Elastin
  • Microscopy
  • Trichrome
  • Vascular Invasion

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Fingerprint

Dive into the research topics of 'Utilizing DUET Imaging for Quantification of Collagen and Elastin as Biomarkers for Studying Disease'. Together they form a unique fingerprint.

Cite this