Abstract
Based an the recent observations that, in a majority of patients with acute leukemia, the 5′ end of the calcitonin gene was hypermethylated and abnormal DNA fragments were observed following HpaII restriction digestion, we have developed a PCR-based method to sensitively detect this abnormal methylation of the calcitonin gene in AML. Applying the concept of competitive PCR, a semi-quantitative correlation was obtained between the amount of hypermethylation and the amount of leukemic cells present. These results suggest that this method will be useful to monitor the amount of tumor cells in bone marrow from patients with AML.
Original language | English (US) |
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Pages (from-to) | 1031-1040 |
Number of pages | 10 |
Journal | Leukemia Research |
Volume | 16 |
Issue number | 10 |
DOIs | |
State | Published - Oct 1992 |
Keywords
- HL-60
- HpaII
- Hypermethylation
- Southern-blot analysis
- acute myelogenous leukemia
- calcitonin gene
- polymerase chain reaction
ASJC Scopus subject areas
- Hematology
- Oncology
- Cancer Research