Abstract
Replacement of autogenous or allograft bones by artificial graft materials represents a growing area of interest in current bone repair strategies. Bioactive ceramics in particulate form, such as Bioglass® (BG) 45S5, stimulate bone mineralization comparable to autologous bone grafts, but have potential issues of particle migration and inflammation. The aim of this study was to employ a chondroitin sulfate- (CS-) based bioadhesive to improve integration of the bioglass (NovaBone® Putty) to prevent particle migration and promote bone regeneration. This BG-CS composite can encapsulate bone marrow (BM) to form a mechanically stable construct, BG-CS-BM. Rheological characterization confirmed the formation of CS-BM hydrogel by reacting the CS-based bioadhesive with the BM. Compared to the bioglass, the BG-CS-BM composite demonstrated a superior capacity to maintain construct integrity under both aqueous and turbulent environments in vitro. After implantation for 4 weeks in a critical-size distal femoral bone defect in a rabbit model, there was significantly greater bone growth in BG-CS-BM as compared to bioglass-only and the empty control. Unlike BG-CS-BM, BG-CS recruited BM in situ from the bone defect. BG-CS demonstrated a similar effect in bone formation but at a comparatively slower rate than BG-CS-BM over 6-weeks' implantation.
Original language | English (US) |
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Pages (from-to) | 235-242 |
Number of pages | 8 |
Journal | Journal of Biomedical Materials Research - Part A |
Volume | 103 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1 2015 |
Keywords
- bioadhesive hydrogel
- bioglass
- bone marrow
- bone regeneration
- chondroitin sulfate
ASJC Scopus subject areas
- Ceramics and Composites
- Biomaterials
- Biomedical Engineering
- Metals and Alloys