Unmasking of human lymphoid cell heteroantigens by neuraminidase treatment1

Steven A. Rosenberg, Barbara A. Plocinik, G. Nicholas Rogentine

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Treatment of human lymphoid tissue-culture cells and fresh human lymphocytes withVibrio choleraeneuraminidase (NASE) underconditions that removed 65% of the cell-surface sialic acid uncoveredsurface structures capable of reacting with substances, probably antibodies, in normal rabbit serum. Three to five times more of these surface componentswere detectable on NASE-treated cultured lymphoid cells than on untreated cells. The antibody in normal rabbit serum could be removed byabsorption with NASE-treated lymphoid cells in the presence of ethylene-diamine tetraethylacetate without lowering complement levels.—J NatCancer Inst 48: 1271-1276, 1972.

Original languageEnglish (US)
Pages (from-to)1271-1276
Number of pages6
JournalJournal of the National Cancer Institute
Volume48
Issue number5
DOIs
StatePublished - May 1972

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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