Uncovering Aberrant Mutant PKA Function with Flow Cytometric FRET

Keyphrases

• Flow Cytometric 100%
• Förster Resonance Energy Transfer 100%
• Living Cells 40%
• Protein-protein Interaction 40%
• Transfer Based 40%
• Flow Cytometer 20%
• Commercially Available 20%
• Functional Impact 20%
• Structural Effect 20%
• Disease-causing mutations 20%
• Multiple Partners 20%
• Cushing's Syndrome 20%
• Binding Curve 20%
• Binding Assay 20%
• Mechanistic Understanding 20%
• Cytometer 20%
• General Applicability 20%
• Relevant Context 20%
• Rapid Construction 20%
• Isothermal Calorimetry 20%
• PKA Catalytic Subunit 20%

Biochemistry, Genetics and Molecular Biology

• Energy Transfer 100%
• Protein-Protein Interaction 40%
• Enzyme Active Site 20%
• Calorimetry 20%