Ultrastructural localization of hydrogen peroxide in experimental autoimmune uveitis

Guey Shuang Wu, David C. Gritz, Lily R. Atalla, David A. Stanforth, Alex Sevanian, Narsing A. Rao

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


One of the most prominent features of S-antigen induced uveitis is the massive infiltration of polymorphonuclear leukocytes (PMNs) and mononuclear cells in the ocular tissues and fluids. These inflammatory cells generate reactive oxygen metabolites as microbicidal agents and release these oxidants into the surrounding tissues. Using the cerium perhydroxide method, we have localized subcellular hydrogen peroxide in various inflamed ocular tissues. Most notably, the positive electron-dense granules were seen in the plasma membranes of PMNs that were infiltrating in the retina and uvea. These deposits were noted also in PMNs located within the extravascular spaces. For the intravascular PMNs, the positive reaction products were seen in much lower concentrations. A direct demonstration of substantial concentrations of hydrogen peroxide in experimental autoimmune uveitis, therefore, suggests the possibility that this reactive metabolite is an inflammatory mediator in this condition.

Original languageEnglish (US)
Pages (from-to)955-961
Number of pages7
JournalCurrent Eye Research
Issue number10
StatePublished - 1992
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience


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