Ultrasensitive enzymatic radioimmunoassay (useria) detects femtomoles of acetylaminofluorene-dna adducts

I. C. Hsu, M. C. Poirier, S. H. Yuspa, R. H. Yolken, C. C. Harris

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


USERIA is a modification of radioimmunoassay and enzyme-linked immunosorbent assay. When compared to these assays, USERIA is 60- and 10-fold, respectively, more sensitive for the detection of ad-ducts in DNA modified by the chemical carcinogen, 2-acetylaminofluorene (AAF). The specific antigen-antibody reaction is enzymatically amplified by an anti-IgG alkaline phosphatase conjugate which converts the substrate, [3H]adenosine-5'-mono-phosphate, to [3H]adenosine. As few as 2 fmol of AAF-DNA adducts can be detected by a competitive USERIA assay and less than 3 fmol of the specific adduct, N-(deoxyguanosine-8-yl)-AAF, can be detected using a non-competitive USERIA approach. The sensitivity of USERIA should aid efforts to measure carcinogen-DNA adducts in biological specimen samples from humans and experimental animals.

Original languageEnglish (US)
Pages (from-to)455-458
Number of pages4
Issue number5
StatePublished - May 1980

ASJC Scopus subject areas

  • Cancer Research


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