Ultrasensitive enzymatic radioimmunoassay (useria) detects femtomoles of acetylaminofluorene-dna adducts

I. C. Hsu; M. C. Poirier; S. H. Yuspa; R. H. Yolken; C. C. Harris

doi:10.1093/carcin/1.5.455

Ultrasensitive enzymatic radioimmunoassay (useria) detects femtomoles of acetylaminofluorene-dna adducts

I. C. Hsu, M. C. Poirier, S. H. Yuspa, R. H. Yolken, C. C. Harris

School of Medicine

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

USERIA is a modification of radioimmunoassay and enzyme-linked immunosorbent assay. When compared to these assays, USERIA is 60- and 10-fold, respectively, more sensitive for the detection of ad-ducts in DNA modified by the chemical carcinogen, 2-acetylaminofluorene (AAF). The specific antigen-antibody reaction is enzymatically amplified by an anti-IgG alkaline phosphatase conjugate which converts the substrate, [³H]adenosine-5'-mono-phosphate, to [³H]adenosine. As few as 2 fmol of AAF-DNA adducts can be detected by a competitive USERIA assay and less than 3 fmol of the specific adduct, N-(deoxyguanosine-8-yl)-AAF, can be detected using a non-competitive USERIA approach. The sensitivity of USERIA should aid efforts to measure carcinogen-DNA adducts in biological specimen samples from humans and experimental animals.

Original language	English (US)
Pages (from-to)	455-458
Number of pages	4
Journal	Carcinogenesis
Volume	1
Issue number	5
DOIs	https://doi.org/10.1093/carcin/1.5.455
State	Published - May 1980

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Cancer Research

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title = "Ultrasensitive enzymatic radioimmunoassay (useria) detects femtomoles of acetylaminofluorene-dna adducts",

abstract = "USERIA is a modification of radioimmunoassay and enzyme-linked immunosorbent assay. When compared to these assays, USERIA is 60- and 10-fold, respectively, more sensitive for the detection of ad-ducts in DNA modified by the chemical carcinogen, 2-acetylaminofluorene (AAF). The specific antigen-antibody reaction is enzymatically amplified by an anti-IgG alkaline phosphatase conjugate which converts the substrate, [3H]adenosine-5'-mono-phosphate, to [3H]adenosine. As few as 2 fmol of AAF-DNA adducts can be detected by a competitive USERIA assay and less than 3 fmol of the specific adduct, N-(deoxyguanosine-8-yl)-AAF, can be detected using a non-competitive USERIA approach. The sensitivity of USERIA should aid efforts to measure carcinogen-DNA adducts in biological specimen samples from humans and experimental animals.",

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AU - Hsu, I. C.

AU - Poirier, M. C.

AU - Yuspa, S. H.

AU - Yolken, R. H.

AU - Harris, C. C.

PY - 1980/5

Y1 - 1980/5

N2 - USERIA is a modification of radioimmunoassay and enzyme-linked immunosorbent assay. When compared to these assays, USERIA is 60- and 10-fold, respectively, more sensitive for the detection of ad-ducts in DNA modified by the chemical carcinogen, 2-acetylaminofluorene (AAF). The specific antigen-antibody reaction is enzymatically amplified by an anti-IgG alkaline phosphatase conjugate which converts the substrate, [3H]adenosine-5'-mono-phosphate, to [3H]adenosine. As few as 2 fmol of AAF-DNA adducts can be detected by a competitive USERIA assay and less than 3 fmol of the specific adduct, N-(deoxyguanosine-8-yl)-AAF, can be detected using a non-competitive USERIA approach. The sensitivity of USERIA should aid efforts to measure carcinogen-DNA adducts in biological specimen samples from humans and experimental animals.

AB - USERIA is a modification of radioimmunoassay and enzyme-linked immunosorbent assay. When compared to these assays, USERIA is 60- and 10-fold, respectively, more sensitive for the detection of ad-ducts in DNA modified by the chemical carcinogen, 2-acetylaminofluorene (AAF). The specific antigen-antibody reaction is enzymatically amplified by an anti-IgG alkaline phosphatase conjugate which converts the substrate, [3H]adenosine-5'-mono-phosphate, to [3H]adenosine. As few as 2 fmol of AAF-DNA adducts can be detected by a competitive USERIA assay and less than 3 fmol of the specific adduct, N-(deoxyguanosine-8-yl)-AAF, can be detected using a non-competitive USERIA approach. The sensitivity of USERIA should aid efforts to measure carcinogen-DNA adducts in biological specimen samples from humans and experimental animals.

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Ultrasensitive enzymatic radioimmunoassay (useria) detects femtomoles of acetylaminofluorene-dna adducts

Abstract

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