@article{01a202aca25a4e2087ae09452c00e426,
title = "Ubiquitination-dependent and -independent repression of target genes by SETDB1 reveal a context-dependent role for its methyltransferase activity during adipogenesis",
abstract = "The lysine methyltransferase SETDB1, an enzyme responsible for methylation of histone H3 at lysine 9, plays a key role in H3K9 tri-methylation-dependent silencing of endogenous retroviruses and developmental genes. Recent studies have shown that ubiquitination of human SETDB1 complements its catalytic activity and the silencing of endogenous retroviruses in human embryonic stem cells. However, it is not known whether SETDB1 ubiquitination is essential for its other major role in epigenetic silencing of developmental gene programs. We previously showed that SETDB1 contributes to the formation of H3K4/H3K9me3 bivalent chromatin domains that keep adipogenic Cebpa and Pparg genes in a poised state for activation and restricts the differentiation potential of pre-adipocytes. Here, we show that ubiquitin-resistant K885A mutant of SETDB1 represses adipogenic genes and inhibits pre-adipocyte differentiation similar to wild-type SETDB1. We show this was due to a compensation mechanism for H3K9me3 chromatin modifications on the Cebpa locus by other H3K9 methyltransferases Suv39H1 and Suv39H2. In contrast, the K885A mutant did not repress other SETDB1 target genes such as Tril and Gas6 suggesting SETDB1 represses its target genes by two mechanisms; one that requires its ubiquitination and another that still requires SETDB1 but not its enzyme activity.",
keywords = "H3K9me3, adipocytes, adipogenesis, bivalent chromatin domains, epigenome, histone methylation, post-translational modifications, ubiquitination",
author = "Ji Zhang and Yoshihiro Matsumura and Yuka Kano and Ayano Yoshida and Takeshi Kawamura and Hiroyuki Hirakawa and Takeshi Inagaki and Toshiya Tanaka and Hiroshi Kimura and Shigeru Yanagi and Kiyoko Fukami and Takefumi Doi and Osborne, {Timothy F.} and Tatsuhiko Kodama and Hiroyuki Aburatani and Juro Sakai",
note = "Funding Information: We thank Dr. Yoichi Shinkai for mSETDB1 plasmid; Dr. Toshio Kitamura for pMXs-puro plasmid; Aoi Uchida, Atena Nishikawa, and Akashi Taguchi-Izumi for technical assistance; Minori Yoshio for secretary assistance; and other members of the Sakai laboratory for helpful discussions. The super-computing resource was provided by Human Genome Center (the University of Tokyo). This study was supported by Grants-in-Aid for Scientific Research to J.S. (16H06390, 20H04835, 21H04826) and Young Scientists to Y.M. (24710225) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, Japan Agency for Medical Research and Development to J.S. (JP20gm1310007) and Takeda Science Foundation to Y.M. Funding Information: We thank Dr. Yoichi Shinkai for mSETDB1 plasmid; Dr. Toshio Kitamura for pMXs‐puro plasmid; Aoi Uchida, Atena Nishikawa, and Akashi Taguchi‐Izumi for technical assistance; Minori Yoshio for secretary assistance; and other members of the Sakai laboratory for helpful discussions. The super‐computing resource was provided by Human Genome Center (the University of Tokyo). This study was supported by Grants‐in‐Aid for Scientific Research to J.S. (16H06390, 20H04835, 21H04826) and Young Scientists to Y.M. (24710225) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, Japan Agency for Medical Research and Development to J.S. (JP20gm1310007) and Takeda Science Foundation to Y.M. Publisher Copyright: {\textcopyright} 2021 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd",
year = "2021",
month = jul,
doi = "10.1111/gtc.12868",
language = "English (US)",
volume = "26",
pages = "513--529",
journal = "Genes to Cells",
issn = "1356-9597",
publisher = "Wiley-Blackwell",
number = "7",
}