Two Rab2 interactors regulate dense-core vesicle maturation

Michael Ailion, Mandy Hannemann, Susan Dalton, Andrea Pappas, Shigeki Watanabe, Jan Hegermann, Qiang Liu, Hsiao Fen Han, Mingyu Gu, Morgan Q. Goulding, Nikhil Sasidharan, Kim Schuske, Patrick Hullett, Stefan Eimer, Erik M. Jorgensen

Research output: Contribution to journalArticlepeer-review

36 Scopus citations


Peptide neuromodulators are released from a unique organelle: the dense-core vesicle. Dense-core vesicles are generated at the trans-Golgi and then sort cargo during maturation before being secreted. To identify proteins that act in this pathway, we performed a genetic screen in Caenorhabditis elegans for mutants defective in dense-core vesicle function. We identified two conserved Rab2-binding proteins: RUND-1, a RUN domain protein, and CCCP-1, a coiled-coil protein. RUND-1 and CCCP-1 colocalize with RAB-2 at the Golgi, and rab-2, rund-1, and cccp-1 mutants have similar defects in sorting soluble and transmembrane dense-core vesicle cargos. RUND-1 also interacts with the Rab2 GAP protein TBC-8 and the BAR domain protein RIC-19, a RAB-2 effector. In summary, a pathway of conserved proteins controls the maturation of dense-core vesicles at the trans-Golgi network.

Original languageEnglish (US)
Pages (from-to)167-180
Number of pages14
Issue number1
StatePublished - Apr 2 2014
Externally publishedYes

ASJC Scopus subject areas

  • General Neuroscience


Dive into the research topics of 'Two Rab2 interactors regulate dense-core vesicle maturation'. Together they form a unique fingerprint.

Cite this