Tritiated thymidine incorporation and cell mediated lympholysis as correlates of acute Graft Versus Host reaction

S. J. Sharkis, D. M. Strong, A. Ahmed, K. W. Sell

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1 Scopus citations

Abstract

The graft-versus-host (GVH) reaction remains a serious consequence of administration of allogeneic immunocompetent cells to an immunosuppressed host even if donors and recipients are matched for major histocompatibility loci. This report describes a murine model for acute GVH reactions. Spleen cells from C3H/He (H-2(k)) mice, after intravenous injection of BALB/c (H-2(d)) spleen cells, were specifically cytotoxic for C3H target cells in vitro 4 days after irradiation and reconstitution. The cells in the recipients apparently are of donor genotype. The spleen cells exhibited rapid proliferation in vitro as measured by the uptake of 3H-TdR. The in vitro proliferation was distinguished from erythropoiesis by an assay of 59Fe incorporation. The kinetics of the in vitro incorporation of 3H-TdR and the in vivo uptake of 59Fe are reported.

Original languageEnglish (US)
Pages (from-to)443-455
Number of pages13
JournalExperimental Hematology
Volume5
Issue number6
StatePublished - Dec 1 1977

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

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