Triggering of RNA interference with RNA-RNA, RNA-DNA, and DNA-RNA nanoparticles

Kirill A. Afonin, Mathias Viard, Ioannis Kagiampakis, Christopher L. Case, Marina A. Dobrovolskaia, Jen Hofmann, Ashlee Vrzak, Maria Kireeva, Wojciech K. Kasprzak, Vineet N. Kewalramani, Bruce A. Shapiro

Research output: Contribution to journalArticlepeer-review

69 Scopus citations


Control over cellular delivery of different functionalities and their synchronized activation is a challenging task. We report several RNA and RNA/DNA-based nanoparticles designed to conditionally activate the RNA interference in various human cells. These nanoparticles allow precise control over their formulation, stability in blood serum, and activation of multiple functionalities. Importantly, interferon and pro-inflammatory cytokine activation assays indicate the significantly lower responses for DNA nanoparticles compared to the RNA counterparts, suggesting greater potential of these molecules for therapeutic use.

Original languageEnglish (US)
Pages (from-to)251-259
Number of pages9
JournalACS Nano
Issue number1
StatePublished - Jan 27 2015


  • FRET
  • RNA and DNA nanoparticles
  • RNA and DNA nanotechnology
  • RNA-DNA hybrids reassociation
  • RNAi

ASJC Scopus subject areas

  • General Materials Science
  • General Engineering
  • General Physics and Astronomy


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