Tricyclic antidepressant radioreceptor assay

Robert B. Innis, Larry Tune, Robert Rock, Raymond Depaulo, David C. U'Prichard, Solomon H. Snyder

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


A radioreceptor assay for tricyclic antideressants described here is based on the ability of these drugs to compete with [3H]-3-quinuclidinyl benzatile (3H-QNB) for binding to muscarinic cholinergic receptors in rat brain membranes. The assay is sensitive, in that it can detect, for example, 2 ng/ml nortriptyline in plasma. Seven plasma samples from depressed patients treated with notriptyline were assayed with the radioreceptor and gas liquid chromatographic methods, and the results for these two methods were almost identical. This assay should be used continously, if at all, in patients treated with other drugs that have potent anticholinergic effects.

Original languageEnglish (US)
Pages (from-to)473-477
Number of pages5
JournalEuropean Journal of Pharmacology
Issue number4
StatePublished - Oct 15 1979


  • Anticholinergic
  • QNB displacement
  • Radioreceptor assay
  • Tricyclic antidepressant

ASJC Scopus subject areas

  • Pharmacology


Dive into the research topics of 'Tricyclic antidepressant radioreceptor assay'. Together they form a unique fingerprint.

Cite this