TY - JOUR
T1 - Translation dynamics of single mRNAs in live cells and neurons
AU - Wu, Bin
AU - Eliscovich, Carolina
AU - Yoon, Young J.
AU - Singer, Robert H.
N1 - Funding Information:
This work was supported by NIH grant NS083085 to R.H.S.
PY - 2016/6/17
Y1 - 2016/6/17
N2 - Translation is the fundamental biological process converting mRNA information into proteins. Single-molecule imaging in live cells has illuminated the dynamics of RNA transcription; however, it is not yet applicable to translation. Here, we report single-molecule imaging of nascent peptides (SINAPS) to assess translation in live cells.The approach provides direct readout of initiation, elongation, and location of translation.We show that mRNAs coding for endoplasmic reticulum (ER) proteins are translated when they encounter the ER membrane. Single-molecule fluorescence recovery after photobleaching provides direct measurement of elongation speed (5 amino acids per second). In primary neurons,mRNAs are translated in proximal dendrites but repressed in distal dendrites and display "bursting" translation.This technology provides a tool with which to address the spatiotemporal translation mechanism of single mRNAs in living cells.
AB - Translation is the fundamental biological process converting mRNA information into proteins. Single-molecule imaging in live cells has illuminated the dynamics of RNA transcription; however, it is not yet applicable to translation. Here, we report single-molecule imaging of nascent peptides (SINAPS) to assess translation in live cells.The approach provides direct readout of initiation, elongation, and location of translation.We show that mRNAs coding for endoplasmic reticulum (ER) proteins are translated when they encounter the ER membrane. Single-molecule fluorescence recovery after photobleaching provides direct measurement of elongation speed (5 amino acids per second). In primary neurons,mRNAs are translated in proximal dendrites but repressed in distal dendrites and display "bursting" translation.This technology provides a tool with which to address the spatiotemporal translation mechanism of single mRNAs in living cells.
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U2 - 10.1126/science.aaf1084
DO - 10.1126/science.aaf1084
M3 - Article
C2 - 27313041
AN - SCOPUS:84966440965
SN - 0036-8075
VL - 352
SP - 1430
EP - 1435
JO - Science
JF - Science
IS - 6292
ER -