@article{5a553875d7734b78a3a53432b4bd6e6b,
title = "Transformation of Different Human Breast Epithelial Cell Types Leads to Distinct Tumor Phenotypes",
abstract = "We investigated the influence of normal cell phenotype on the neoplastic phenotype by comparing tumors derived from two different normal human mammary epithelial cell populations, one of which was isolated using a new culture medium. Transformation of these two cell populations with the same set of genetic elements yielded cells that formed tumor xenografts exhibiting major differences in histopathology, tumorigenicity, and metastatic behavior. While one cell type (HMECs) yielded squamous cell carcinomas, the other cell type (BPECs) yielded tumors closely resembling human breast adenocarcinomas. Transformed BPECs gave rise to lung metastases and were up to 104-fold more tumorigenic than transformed HMECs, which are nonmetastatic. Hence, the pre-existing differences between BPECs and HMECs strongly influence the phenotypes of their transformed derivatives.",
keywords = "CELLBIO, CELLCYCLE",
author = "Ince, {Tan A.} and Richardson, {Andrea L.} and Bell, {George W.} and Maki Saitoh and Samuel Godar and Karnoub, {Antoine E.} and Iglehart, {James D.} and Weinberg, {Robert A.}",
note = "Funding Information: We thank J. Slingerland, C.P. Crum, G.L. Mutter, B.J. Quade, D.P. Silver, C. Kuperwasser, J. Yang, I. Ben-Porath, S. McAllister, K. Hartwell, H. Vaziri, and S.K. Dessain for critical reading of the manuscript, stimulating discussions, and support. We also thank Ferenc Reinhardt for excellent technical help with mouse xenograft experiments, Terri Woo and the Quigley laboratory at Brigham and Women's Hospital for their assistance with immunohistochemical stains, as well as Ed Fox and the DF/BWH Cancer Center Microarray Core Lab and Jennifer Love at the Whitehead Institute Center for Microarray Technology. R.A.W. is an American Cancer Society Research Professor and a Daniel K. Ludwig Foundation Cancer Research Professor. This work was funded largely by grants from the Breast Cancer Research Foundation, NY (T.A.I. and R.A.W.), with additional support from a KO8 award (CA 092013) from the National Cancer Institute (T.A.I.) and from the Dana-Farber/Harvard SPORE in Breast Cancer (CA 089393) Career Development Award (T.A.I.). ",
year = "2007",
month = aug,
day = "14",
doi = "10.1016/j.ccr.2007.06.013",
language = "English (US)",
volume = "12",
pages = "160--170",
journal = "Cancer cell",
issn = "1535-6108",
publisher = "Cell Press",
number = "2",
}