TY - JOUR
T1 - Transfection of rat kidney with human 15-lipoxygenase suppresses inflammation and preserves function in experimental glomerulonephritis
AU - Munger, Karen A.
AU - Montero, Angel
AU - Fukunaga, Megumu
AU - Uda, Susumu
AU - Yura, Takafumi
AU - Imai, Enyu
AU - Kaneda, Yasufumi
AU - Valdivielso, José M.
AU - Badr, Kamal F.
PY - 1999/11/9
Y1 - 1999/11/9
N2 - The human 15-lipoxygenase (15-LO) gene was transfected into rat kidneys in vivo via intra-renal arterial injection. Three days later, acute (passive) or accelerated forms of antiglomerular basement membrane antibody-mediated glomerulonephritis were induced in transfected and nontransfected or sham- transfected controls. Studies of glomerular functions (filtration and protein excretion) and ex vivo glomerular leukotriene B4 biosynthesis at 3 hr, and up to 4 days, after induction of nephritis revealed preservation or normalization of these parameters in transfected kidneys that expressed human 15-LO mRNA and mature protein, but not in contralateral control kidneys or sham-transfected animals. The results provide in vivo-derived data supporting a direct antiinflammatory role for 15-LO during immune-mediated tissue injury.
AB - The human 15-lipoxygenase (15-LO) gene was transfected into rat kidneys in vivo via intra-renal arterial injection. Three days later, acute (passive) or accelerated forms of antiglomerular basement membrane antibody-mediated glomerulonephritis were induced in transfected and nontransfected or sham- transfected controls. Studies of glomerular functions (filtration and protein excretion) and ex vivo glomerular leukotriene B4 biosynthesis at 3 hr, and up to 4 days, after induction of nephritis revealed preservation or normalization of these parameters in transfected kidneys that expressed human 15-LO mRNA and mature protein, but not in contralateral control kidneys or sham-transfected animals. The results provide in vivo-derived data supporting a direct antiinflammatory role for 15-LO during immune-mediated tissue injury.
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U2 - 10.1073/pnas.96.23.13375
DO - 10.1073/pnas.96.23.13375
M3 - Article
C2 - 10557328
AN - SCOPUS:0033539695
SN - 0027-8424
VL - 96
SP - 13375
EP - 13380
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 23
ER -