@inbook{7c921740e3fa47d4bef65720c40bcf89,
title = "Transcriptional regulation using the Q system in transgenic zebrafish",
abstract = "Methods to label cell populations selectively or to modify their gene expression are critical tools in the study of developmental or physiological processes in vivo. A variety of approaches have been applied to the zebrafish model, capitalizing on Tol2 transposition to generate transgenic lines with high efficiency. Here we describe the adoption of the Q system of Neurospora crassa, which includes the QF transcription factor and the upstream activating sequence (QUAS) to which it binds. These components function as a bipartite regulatory system similar to that of yeast Gal4/UAS, producing robust expression in transient assays of zebrafish embryos injected with plasmids and in stable transgenic lines. An important advantage, however, is that QUAS-regulated transgenes appear far less susceptible to transcriptional silencing even after seven generations. This chapter describes some of the Q system reagents that have been developed for zebrafish, as well as the use of the QF transcription factor for isolation of tissue-specific driver lines from gene/enhancer trap screens. Additional strategies successfully implemented in invertebrate models, such as a truncated QF transcription factor (QF2) or the reassembly of a split QF, are also discussed. The provided information, and available Gateway-based vectors, should enable those working with the zebrafish model to implement the Q system with minimal effort or to use it in combination with Gal4, Cre, or other regulatory systems for further refinement of transcriptional control.",
keywords = "Bipartite system, Gal4, Transcriptional regulation, Transgenesis, qalocus",
author = "A. Ghosh and Halpern, {M. E.}",
note = "Publisher Copyright: {\textcopyright} 2016 Elsevier Inc.",
year = "2016",
doi = "10.1016/bs.mcb.2016.05.001",
language = "English (US)",
isbn = "9780128034743",
series = "Methods in Cell Biology",
publisher = "Academic Press Inc.",
pages = "205--218",
editor = "Zon, {Leonard I.} and Monte Westerfield and {William Detrich}, H.",
booktitle = "The Zebrafish Genetics, Genomics, and Transcriptomics, 2016",
}