@inbook{7c921740e3fa47d4bef65720c40bcf89,
title = "Transcriptional regulation using the Q system in transgenic zebrafish",
abstract = "Methods to label cell populations selectively or to modify their gene expression are critical tools in the study of developmental or physiological processes in vivo. A variety of approaches have been applied to the zebrafish model, capitalizing on Tol2 transposition to generate transgenic lines with high efficiency. Here we describe the adoption of the Q system of Neurospora crassa, which includes the QF transcription factor and the upstream activating sequence (QUAS) to which it binds. These components function as a bipartite regulatory system similar to that of yeast Gal4/UAS, producing robust expression in transient assays of zebrafish embryos injected with plasmids and in stable transgenic lines. An important advantage, however, is that QUAS-regulated transgenes appear far less susceptible to transcriptional silencing even after seven generations. This chapter describes some of the Q system reagents that have been developed for zebrafish, as well as the use of the QF transcription factor for isolation of tissue-specific driver lines from gene/enhancer trap screens. Additional strategies successfully implemented in invertebrate models, such as a truncated QF transcription factor (QF2) or the reassembly of a split QF, are also discussed. The provided information, and available Gateway-based vectors, should enable those working with the zebrafish model to implement the Q system with minimal effort or to use it in combination with Gal4, Cre, or other regulatory systems for further refinement of transcriptional control.",
keywords = "Bipartite system, Gal4, Transcriptional regulation, Transgenesis, qalocus",
author = "A. Ghosh and Halpern, {M. E.}",
note = "Funding Information: We thank Jean Michael Chanchu, Tyler Harvey, Ayse Keskus, Michelle Macurak, and Estela Monge for their expert technical assistance in developing reagents and providing results described in this chapter. Abigyna Subedi and Mary Goll were instrumental in initiating this project and Chris Potter has generously provided plasmids and advice. We are grateful to Shannon Fisher, Koichi Kawakami, Kristen Kwan, Michael Parsons, and Kang Shen for sharing plasmids. The zebrafish Q project is supported by the National Institute of Child Health and Human Development (4R01HD078220). Publisher Copyright: {\textcopyright} 2016 Elsevier Inc.",
year = "2016",
doi = "10.1016/bs.mcb.2016.05.001",
language = "English (US)",
isbn = "9780128034743",
series = "Methods in Cell Biology",
publisher = "Academic Press Inc.",
pages = "205--218",
editor = "Zon, {Leonard I.} and Monte Westerfield and {William Detrich}, H.",
booktitle = "The Zebrafish Genetics, Genomics, and Transcriptomics, 2016",
}