TY - JOUR
T1 - Transcriptional analysis of the adenovirus-5 EIII promoter
T2 - Absence of sequence specificity for stimulation by EIa gene products
AU - Leff, Todd
AU - Corden, Jeff
AU - Elkaim, René
AU - Sassone-Corsi, Paolo
N1 - Funding Information:
ACKNOWLEDGEMENTS We thank Adrien Staub for the preparation of the EIII-ol1gonucle-otide; Drs. P. Chambon and C. Goding for helpful 1 discussions ; C. Hauss for excellent technical assistance ; M. Acker and B. Augsburger for growing cells, and C. Aron, C. Werle and B. Boulay for assistance 1n preparing the manuscript. T. Leff was as a recipient of a National Science Foundation/ Centre National de la Recherche Sc1ent1f1que exchange fellowship. This work was supported by the CNRS (ATP 3582), the INSERM (PRC 124026), the Fondation pour la Recherche Medicale, the M1n1ste>e de l'lndustrie et de la Recherche (82V1283) and the Association pour le Developpement de la Recherche sur le Cancer.
PY - 1985/2/25
Y1 - 1985/2/25
N2 - To identify the adenovirus-5 EIII promoter sequences that are involved in basal level of transcription, a series of promoter deletion mutants were analyzed in vivo by transfection into HeLa cells and in vitro using a HeLa whole cell extract system. Three regions within the EIII promoter were shown to be important for efficient transcription : the TATA sequence, an upstream element centered at -55/-57, and an additional element located between -111 and -233. In vivo transcriptional analysis of EIII promoter deletions in the presence of adenovirus EIa gene products have demonstrated that the same three regions are required for EIa- stimulated transcription. We conclude that there is no sequence element in the EIII promoter between -15 and -233 that is uniquely required for the stimulation of EIII transcription by EIa gene products.
AB - To identify the adenovirus-5 EIII promoter sequences that are involved in basal level of transcription, a series of promoter deletion mutants were analyzed in vivo by transfection into HeLa cells and in vitro using a HeLa whole cell extract system. Three regions within the EIII promoter were shown to be important for efficient transcription : the TATA sequence, an upstream element centered at -55/-57, and an additional element located between -111 and -233. In vivo transcriptional analysis of EIII promoter deletions in the presence of adenovirus EIa gene products have demonstrated that the same three regions are required for EIa- stimulated transcription. We conclude that there is no sequence element in the EIII promoter between -15 and -233 that is uniquely required for the stimulation of EIII transcription by EIa gene products.
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U2 - 10.1093/nar/13.4.1209
DO - 10.1093/nar/13.4.1209
M3 - Article
C2 - 4000937
AN - SCOPUS:0022431869
SN - 0305-1048
VL - 13
SP - 1209
EP - 1221
JO - Nucleic acids research
JF - Nucleic acids research
IS - 4
ER -