Transcriptional activator nuclear factor I stimulates the replication of SV40 minichromosomes in vivo and in vitro

Linzhao Cheng; Thomas J. Kelly

doi:10.1016/0092-8674(89)90037-8

Transcriptional activator nuclear factor I stimulates the replication of SV40 minichromosomes in vivo and in vitro

Linzhao Cheng, Thomas J. Kelly

School of Medicine

Research output: Contribution to journal › Article › peer-review

106 Scopus citations

Abstract

SV40 DNA replication in vivo is greatly stimulated by cis-acting transcriptional elements. We studied a model viral chromosome containing a single binding site for the cellular transcriptional activator, nuclear factor I ( NF-I CTF), located adjacent to the replication origin. The presence of the NF-I recognition site increased replication efficiency over 20-fold in vivo. Purified NF-I had little effect on the replication efficiency in the standard SV40 cell-free system when the template was introduced as naked DNA. However, NF-I specifically prevented the repression of DNA replication that occurred when the template was preassembled into chromatin. Our data support a model in which the binding of a transcriptional activator perturbs the local distribution of nucleosomes, thereby increasing the accessibility of the origin region.

Original language	English (US)
Pages (from-to)	541-551
Number of pages	11
Journal	Cell
Volume	59
Issue number	3
DOIs	https://doi.org/10.1016/0092-8674(89)90037-8
State	Published - Nov 3 1989

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1016/0092-8674(89)90037-8

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@article{b9481235c32345cd83896cc2f464d283,

title = "Transcriptional activator nuclear factor I stimulates the replication of SV40 minichromosomes in vivo and in vitro",

abstract = "SV40 DNA replication in vivo is greatly stimulated by cis-acting transcriptional elements. We studied a model viral chromosome containing a single binding site for the cellular transcriptional activator, nuclear factor I ( NF-I CTF), located adjacent to the replication origin. The presence of the NF-I recognition site increased replication efficiency over 20-fold in vivo. Purified NF-I had little effect on the replication efficiency in the standard SV40 cell-free system when the template was introduced as naked DNA. However, NF-I specifically prevented the repression of DNA replication that occurred when the template was preassembled into chromatin. Our data support a model in which the binding of a transcriptional activator perturbs the local distribution of nucleosomes, thereby increasing the accessibility of the origin region.",

author = "Linzhao Cheng and Kelly, {Thomas J.}",

note = "Funding Information: We would like to thank Dr. Bret Jesse and Hui Zhang for help in preparing Xenopus oocyte extract and Dr. Edward O{\textquoteright}Nell for the generous supplement of purified NF-I protein. We are grateful to Drs. David Weinberg, Joachim Li, Marc Wold, and other colleagues in the laboratory for fruitful discussion. This work was supported by grant CA40414-03 from the National Institutes of Health.",

year = "1989",

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doi = "10.1016/0092-8674(89)90037-8",

language = "English (US)",

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pages = "541--551",

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T1 - Transcriptional activator nuclear factor I stimulates the replication of SV40 minichromosomes in vivo and in vitro

AU - Cheng, Linzhao

AU - Kelly, Thomas J.

N1 - Funding Information: We would like to thank Dr. Bret Jesse and Hui Zhang for help in preparing Xenopus oocyte extract and Dr. Edward O’Nell for the generous supplement of purified NF-I protein. We are grateful to Drs. David Weinberg, Joachim Li, Marc Wold, and other colleagues in the laboratory for fruitful discussion. This work was supported by grant CA40414-03 from the National Institutes of Health.

PY - 1989/11/3

Y1 - 1989/11/3

N2 - SV40 DNA replication in vivo is greatly stimulated by cis-acting transcriptional elements. We studied a model viral chromosome containing a single binding site for the cellular transcriptional activator, nuclear factor I ( NF-I CTF), located adjacent to the replication origin. The presence of the NF-I recognition site increased replication efficiency over 20-fold in vivo. Purified NF-I had little effect on the replication efficiency in the standard SV40 cell-free system when the template was introduced as naked DNA. However, NF-I specifically prevented the repression of DNA replication that occurred when the template was preassembled into chromatin. Our data support a model in which the binding of a transcriptional activator perturbs the local distribution of nucleosomes, thereby increasing the accessibility of the origin region.

AB - SV40 DNA replication in vivo is greatly stimulated by cis-acting transcriptional elements. We studied a model viral chromosome containing a single binding site for the cellular transcriptional activator, nuclear factor I ( NF-I CTF), located adjacent to the replication origin. The presence of the NF-I recognition site increased replication efficiency over 20-fold in vivo. Purified NF-I had little effect on the replication efficiency in the standard SV40 cell-free system when the template was introduced as naked DNA. However, NF-I specifically prevented the repression of DNA replication that occurred when the template was preassembled into chromatin. Our data support a model in which the binding of a transcriptional activator perturbs the local distribution of nucleosomes, thereby increasing the accessibility of the origin region.

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AN - SCOPUS:0024357459

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EP - 551

JO - Cell

JF - Cell

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Transcriptional activator nuclear factor I stimulates the replication of SV40 minichromosomes in vivo and in vitro

Abstract

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