TY - GEN
T1 - Towards single-molecule diagnostics using microfluidic manipulation and quantum dot nanosensors
AU - Yeh, Hsin Chih
AU - Puleo, Christopher M.
AU - Ho, Yi Ping
AU - Wang, Tza Huei
PY - 2007
Y1 - 2007
N2 - In this report, we review several single-molecule detection (SMD) methods and newly developed nanocrystal-mediated single-fluorophore strategies for ultrasensitive and specific analysis of genomic sequences. These include techniques, such as quantum dot (QD)-mediated fluorescence resonance energy transfer (FRET) technology and dual-color fluorescence coincidence and colocalization analysis, which allow separation-free detection of low-abundance DNA sequences and mutational analysis of oncogenes. Microfluidic approaches developed for use with single-molecule detection to achieve rapid, low-volume, and quantitative analysis of nucleic acids, such as electrokinetic manipulation of single molecules and confinement of sub-nanoliter samples using microfluidic networks integrated with valves, are also discussed.
AB - In this report, we review several single-molecule detection (SMD) methods and newly developed nanocrystal-mediated single-fluorophore strategies for ultrasensitive and specific analysis of genomic sequences. These include techniques, such as quantum dot (QD)-mediated fluorescence resonance energy transfer (FRET) technology and dual-color fluorescence coincidence and colocalization analysis, which allow separation-free detection of low-abundance DNA sequences and mutational analysis of oncogenes. Microfluidic approaches developed for use with single-molecule detection to achieve rapid, low-volume, and quantitative analysis of nucleic acids, such as electrokinetic manipulation of single molecules and confinement of sub-nanoliter samples using microfluidic networks integrated with valves, are also discussed.
UR - https://www.scopus.com/pages/publications/37249078600
UR - https://www.scopus.com/pages/publications/37249078600#tab=citedBy
U2 - 10.1115/ICNMM2007-30213
DO - 10.1115/ICNMM2007-30213
M3 - Conference contribution
AN - SCOPUS:37249078600
SN - 079184272X
SN - 9780791842720
T3 - Proceedings of the 5th International Conference on Nanochannels, Microchannels and Minichannels, ICNMM2007
SP - 1133
EP - 1140
BT - Proceedings of the 5th International Conference on Nanochannels, Microchannels and Minichannels, ICNMM2007
T2 - 5th International Conference on Nanochannels, Microchannels and Minichannels, ICNMM2007
Y2 - 18 June 2007 through 20 June 2007
ER -