Towards an HIV-1 cure: Measuring the latent reservoir

Katherine M. Bruner; Nina N. Hosmane; Robert F. Siliciano

doi:10.1016/j.tim.2015.01.013

Towards an HIV-1 cure: Measuring the latent reservoir

Katherine M. Bruner, Nina N. Hosmane, Robert F. Siliciano

Howard Hughes Medical Institution

Research output: Contribution to journal › Review article › peer-review

140 Scopus citations

Abstract

The latent reservoir (LR) of HIV-1 in resting memory CD4⁺ T cells serves as a major barrier to curing HIV-1 infection. While many PCR- and culture-based assays have been used to measure the size of the LR, correlation between results of different assays is poor and recent studies indicate that no available assay provides an accurate measurement of reservoir size. The discrepancies between assays are a hurdle to clinical trials that aim to measure the efficacy of HIV-1 eradication strategies. Here we describe the advantages and disadvantages of various approaches to measuring the LR.

Original language	English (US)
Pages (from-to)	192-203
Number of pages	12
Journal	Trends in Microbiology
Volume	23
Issue number	4
DOIs	https://doi.org/10.1016/j.tim.2015.01.013
State	Published - Apr 1 2015

Keywords

Assays
Cure
HIV-1
Latency

ASJC Scopus subject areas

Microbiology
Microbiology (medical)
Virology
Infectious Diseases

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10.1016/j.tim.2015.01.013

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title = "Towards an HIV-1 cure: Measuring the latent reservoir",

abstract = "The latent reservoir (LR) of HIV-1 in resting memory CD4+ T cells serves as a major barrier to curing HIV-1 infection. While many PCR- and culture-based assays have been used to measure the size of the LR, correlation between results of different assays is poor and recent studies indicate that no available assay provides an accurate measurement of reservoir size. The discrepancies between assays are a hurdle to clinical trials that aim to measure the efficacy of HIV-1 eradication strategies. Here we describe the advantages and disadvantages of various approaches to measuring the LR.",

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AU - Siliciano, Robert F.

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AB - The latent reservoir (LR) of HIV-1 in resting memory CD4+ T cells serves as a major barrier to curing HIV-1 infection. While many PCR- and culture-based assays have been used to measure the size of the LR, correlation between results of different assays is poor and recent studies indicate that no available assay provides an accurate measurement of reservoir size. The discrepancies between assays are a hurdle to clinical trials that aim to measure the efficacy of HIV-1 eradication strategies. Here we describe the advantages and disadvantages of various approaches to measuring the LR.

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KW - Cure

KW - HIV-1

KW - Latency

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Towards an HIV-1 cure: Measuring the latent reservoir

Abstract

Keywords

ASJC Scopus subject areas

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