@article{6aa3cb82dbb248d6adde1ee54f7af997,
title = "Tn7 transposition in vitro proceeds through an excised transposon intermediate generated by staggered breaks in DNA",
abstract = "We have developed a cell-free system in which the bacterial transposon Tn7 inserts at high frequency into its preferred target site in the Escherichia coil chromosome, attTn7; Tn7 transposition in vitro requires ATP and Tn7-encoded proteins. Tn7 transposes via a cut and paste mechanism in which the element is excised from the donor DNA by staggered double-strand breaks and then inserted into attTn7 by the joining of 3′ transposon ends to 5′ target ends. Neither recombination Intermediates nor products are observed in the absence of any protein component or DNA substrate. Thus, we suggest that Tn7 transposition occurs in a nucleoprotein complex containing several proteins and the substrate DNAs and that recognition of attTn7 within this complex provokes strand cleavages at the Tn7 ends.",
author = "Roland Bainton and Pascal Gamas and Craig, {Nancy L.}",
note = "Funding Information: Acknowledgments We thank other members of the laboratory for materials and advice: Karina Orle for tns plasmids and anti-Tns antibodies, Lidia Arciszew-ska for TnsB, and Ken Kubo for TnsD fractions. Glenn Gloor, George Chaconas, and Pat Brown also provided useful methods. We are especially grateful to Rudi Grosschedl, Ann Hagemann, Donald Morisato, Anne Stellwagen, Evi Strauss, and Candy Waddell for their comments on the manuscript. R. B. was supported by funds from the Medical Scientist Training Program (NIH-MSTP-GM0 7618) Merck Sharp and Dohme Research Laboratories, the UCSF Department of Biochemistry Tompkins Memorial Fund, and the UCSF Program in Biological Sciences. P. G. was supported by funds from the Weingarten Foundation and the NIH-CNRS program, and by a NATO fellowship. The work was supported by a grant from the National Institute of Allergy and Infectious Disease to N. L. C. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with USC 18 Section 1734 solely to indicate this fact. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",
year = "1991",
month = may,
day = "31",
doi = "10.1016/0092-8674(91)90388-F",
language = "English (US)",
volume = "65",
pages = "805--816",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "5",
}