Tn7 transposes proximal to DNA double-strand breaks and into regions where chromosomal DNA replication terminates

Joseph E. Peters; Nancy L. Craig

doi:10.1016/S1097-2765(00)00056-3

Tn7 transposes proximal to DNA double-strand breaks and into regions where chromosomal DNA replication terminates

Joseph E. Peters, Nancy L. Craig

School of Medicine

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

We report that the bacterial transposon Tn7 can preferentially transpose into regions where chromosomal DNA replication terminates. DNA double-strand breaks are associated with the termination of chromosomal replication; therefore, we directly tested the effect of DNA breaks on Tn7 transposition. When DNA double-strand breaks are induced at specific sites in the chromosome, Tn7 transposition is stimulated and insertions are directed proximal to the induced break. The targeting preference for the terminus of replication and DNA double-strand breaks is dependent on the Tn7-encoded protein TnsE. The results presented in this study could also explain the previous observation that Tn7 is attracted to events associated with conjugal DNA replication during plasmid DNA transfer.

Original language	English (US)
Pages (from-to)	573-582
Number of pages	10
Journal	Molecular cell
Volume	6
Issue number	3
DOIs	https://doi.org/10.1016/S1097-2765(00)00056-3
State	Published - 2000

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/S1097-2765(00)00056-3

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@article{c2c1a791372743abbcdda0e1d23cad68,

title = "Tn7 transposes proximal to DNA double-strand breaks and into regions where chromosomal DNA replication terminates",

abstract = "We report that the bacterial transposon Tn7 can preferentially transpose into regions where chromosomal DNA replication terminates. DNA double-strand breaks are associated with the termination of chromosomal replication; therefore, we directly tested the effect of DNA breaks on Tn7 transposition. When DNA double-strand breaks are induced at specific sites in the chromosome, Tn7 transposition is stimulated and insertions are directed proximal to the induced break. The targeting preference for the terminus of replication and DNA double-strand breaks is dependent on the Tn7-encoded protein TnsE. The results presented in this study could also explain the previous observation that Tn7 is attracted to events associated with conjugal DNA replication during plasmid DNA transfer.",

author = "Peters, {Joseph E.} and Craig, {Nancy L.}",

note = "Funding Information: We are grateful to David Haniford, Tom Hill, Jean-Paul Louran, Colin Manoil, John Roth, Nancy Trun, and George Weinstock for providing strains, plasmids, and advice. This work was supported by NIH training grant 5T32CA09139 (J. E. P.), by the NSF under grant DBI-9750072 (J. E. P.), and by the NIH under grant GM53824 to N.L.C. N.L.C. is an investigator in the Howard Hughes Medical Institute.",

year = "2000",

doi = "10.1016/S1097-2765(00)00056-3",

language = "English (US)",

volume = "6",

pages = "573--582",

journal = "Molecular cell",

issn = "1097-2765",

publisher = "Cell Press",

number = "3",

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TY - JOUR

T1 - Tn7 transposes proximal to DNA double-strand breaks and into regions where chromosomal DNA replication terminates

AU - Peters, Joseph E.

AU - Craig, Nancy L.

N1 - Funding Information: We are grateful to David Haniford, Tom Hill, Jean-Paul Louran, Colin Manoil, John Roth, Nancy Trun, and George Weinstock for providing strains, plasmids, and advice. This work was supported by NIH training grant 5T32CA09139 (J. E. P.), by the NSF under grant DBI-9750072 (J. E. P.), and by the NIH under grant GM53824 to N.L.C. N.L.C. is an investigator in the Howard Hughes Medical Institute.

PY - 2000

Y1 - 2000

N2 - We report that the bacterial transposon Tn7 can preferentially transpose into regions where chromosomal DNA replication terminates. DNA double-strand breaks are associated with the termination of chromosomal replication; therefore, we directly tested the effect of DNA breaks on Tn7 transposition. When DNA double-strand breaks are induced at specific sites in the chromosome, Tn7 transposition is stimulated and insertions are directed proximal to the induced break. The targeting preference for the terminus of replication and DNA double-strand breaks is dependent on the Tn7-encoded protein TnsE. The results presented in this study could also explain the previous observation that Tn7 is attracted to events associated with conjugal DNA replication during plasmid DNA transfer.

AB - We report that the bacterial transposon Tn7 can preferentially transpose into regions where chromosomal DNA replication terminates. DNA double-strand breaks are associated with the termination of chromosomal replication; therefore, we directly tested the effect of DNA breaks on Tn7 transposition. When DNA double-strand breaks are induced at specific sites in the chromosome, Tn7 transposition is stimulated and insertions are directed proximal to the induced break. The targeting preference for the terminus of replication and DNA double-strand breaks is dependent on the Tn7-encoded protein TnsE. The results presented in this study could also explain the previous observation that Tn7 is attracted to events associated with conjugal DNA replication during plasmid DNA transfer.

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DO - 10.1016/S1097-2765(00)00056-3

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SN - 1097-2765

VL - 6

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EP - 582

JO - Molecular cell

JF - Molecular cell

IS - 3

ER -

Tn7 transposes proximal to DNA double-strand breaks and into regions where chromosomal DNA replication terminates

Abstract

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