Three related centromere proteins are absent from the inactive centromere of a stable isodicentric chromosome

William C. Earnshaw; Barbara R. Migeon

doi:10.1007/BF00329812

Three related centromere proteins are absent from the inactive centromere of a stable isodicentric chromosome

William C. Earnshaw, Barbara R. Migeon

School of Medicine

Research output: Contribution to journal › Article › peer-review

181 Scopus citations

Abstract

We developed an aqueous spreading procedure that permits simultaneous analysis of human chromosomes by Q-banding and indirect immunofluorescence. Using this methodology and anticentromere antibodies from an autoimmune patient we compared the active and inactive centromeres of an isodicentric X chromosome. We show that a family of structurally related human centromere proteins (CENP-A, CENP-B, and CENP-C) is detectable only at the active centromere. These antigens therefore may be regarded both as morphological and functional markers for active centromeres.

Original language	English (US)
Pages (from-to)	290-296
Number of pages	7
Journal	Chromosoma
Volume	92
Issue number	4
DOIs	https://doi.org/10.1007/BF00329812
State	Published - Aug 1985

ASJC Scopus subject areas

Genetics
Genetics(clinical)

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abstract = "We developed an aqueous spreading procedure that permits simultaneous analysis of human chromosomes by Q-banding and indirect immunofluorescence. Using this methodology and anticentromere antibodies from an autoimmune patient we compared the active and inactive centromeres of an isodicentric X chromosome. We show that a family of structurally related human centromere proteins (CENP-A, CENP-B, and CENP-C) is detectable only at the active centromere. These antigens therefore may be regarded both as morphological and functional markers for active centromeres.",

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year = "1985",

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T1 - Three related centromere proteins are absent from the inactive centromere of a stable isodicentric chromosome

AU - Earnshaw, William C.

AU - Migeon, Barbara R.

PY - 1985/8

Y1 - 1985/8

N2 - We developed an aqueous spreading procedure that permits simultaneous analysis of human chromosomes by Q-banding and indirect immunofluorescence. Using this methodology and anticentromere antibodies from an autoimmune patient we compared the active and inactive centromeres of an isodicentric X chromosome. We show that a family of structurally related human centromere proteins (CENP-A, CENP-B, and CENP-C) is detectable only at the active centromere. These antigens therefore may be regarded both as morphological and functional markers for active centromeres.

AB - We developed an aqueous spreading procedure that permits simultaneous analysis of human chromosomes by Q-banding and indirect immunofluorescence. Using this methodology and anticentromere antibodies from an autoimmune patient we compared the active and inactive centromeres of an isodicentric X chromosome. We show that a family of structurally related human centromere proteins (CENP-A, CENP-B, and CENP-C) is detectable only at the active centromere. These antigens therefore may be regarded both as morphological and functional markers for active centromeres.

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JO - Chromosoma

JF - Chromosoma

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ER -

Three related centromere proteins are absent from the inactive centromere of a stable isodicentric chromosome

Abstract

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