Three-dimensional printing of tissue phantoms for biophotonic imaging

Jianting Wang, James Coburn, Chia Pin Liang, Nicholas Woolsey, Jessica C. Ramella-Roman, Yu Chen, T. Joshua Pfefer

Research output: Contribution to journalArticlepeer-review

42 Scopus citations


We have investigated the potential of tissue phantoms fabricated with thermosoftening- and photopolymerizationbased three-dimensional (3D) printers for use in evaluation of biophotonic imaging systems. The optical properties of printed polymer samples were measured and compared to biological tissues. Phantoms with subsurface channels as small as 0.2 mm in diameter were fabricated and imaged with microscopy, x-ray microtomography, and optical coherence tomography to characterize morphology. These phantoms were then implemented to evaluate the penetration depth of a hyperspectral reflectance imaging system used in conjunction with a near-infrared contrast agent. Results indicated that 3D printing may provide a suitable platform for performance testing in biophotonics, although subsurface imaging is critical to mitigate printer-to-printer variability in matrix homogeneity and feature microstructure.

Original languageEnglish (US)
Pages (from-to)3010-3013
Number of pages4
JournalOptics Letters
Issue number10
StatePublished - May 15 2014
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Medicine(all)


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