Three-dimensional automated reporter quantification (3D-ARQ) technology enables quantitative screening in retinal organoids

M. Natalia Vergara, Miguel Flores-Bellver, Silvia Aparicio-Domingo, Minda McNally, Karl J. Wahlin, Meera T. Saxena, Jeff S. Mumm, M. Valeria Canto-Soler

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


The advent of stem cell-derived retinal organoids has brought forth unprecedented opportunities for developmental and physiological studies, while presenting new therapeutic promise for retinal degenerative diseases. From a translational perspective, organoid systems provide exciting new prospects for drug discovery, offering the possibility to perform compound screening in a three-dimensional (3D) human tissue context that resembles the native histoarchitecture and to some extent recapitulates cellular interactions. However, inherent variability issues and a general lack of robust quantitative technologies for analyzing organoids on a large scale pose severe limitations for their use in translational applications. To address this need, we have developed a screening platform that enables accurate quantification of fluorescent reporters in complex human iPSCderived retinal organoids. This platform incorporates a fluorescence microplate reader that allows xyz-dimensional detection and finetuned wavelength selection.We have established optimal parameters for fluorescent reporter signal detection, devised methods to compensate for organoid size variability, evaluated performance and sensitivity parameters, and validated this technology for functional applications.

Original languageEnglish (US)
Pages (from-to)3698-3705
Number of pages8
JournalDevelopment (Cambridge)
Issue number20
StatePublished - Oct 15 2017


  • 3D-ARQ
  • Fluorescence reporter quantification
  • Human
  • Retinal organoids
  • Screening

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology


Dive into the research topics of 'Three-dimensional automated reporter quantification (3D-ARQ) technology enables quantitative screening in retinal organoids'. Together they form a unique fingerprint.

Cite this