TY - GEN
T1 - Three-dimensional angular domain optical projection tomography
AU - Ng, Eldon
AU - Vasefi, Fartash
AU - Roumeliotis, Michael
AU - Kaminska, Bozena
AU - Carson, Jeffrey J.L.
PY - 2011
Y1 - 2011
N2 - Angular Domain Imaging (ADI) has been previously demonstrated to generate projection images of attenuating targets embedded within a turbid medium. The imaging system employs a silicon micro-tunnel array positioned between the sample and the detection system to reject scattered photons that have deviated from the initial propagation direction and to select for ballistic and quasi-ballistic photons that have retained their forward trajectory. Two dimensional tomographic images can be reconstructed from ADI projections collected at a multitude of angles. The objective of this work was to extend the system to three dimensions by collecting several tomographic images and stacking the reconstructed slices to generate a three dimensional volume representative of the imaging target. A diode laser (808nm, CW) with a beam expander was used to illuminate the sample cuvette. An Angular Filter Array (AFA) of 80 μm × 80 μm square-shaped tunnels 2 cm in length was used to select for image forming quasi-ballistic photons. Images were detected with a linear CCD. Our approach was to use a SCARA robot to rotate and translate the sample to collect sufficient projections to reconstruct a three dimensional volume. A custom designed 3D target consisting of 4 truncated cones was imaged and reconstructed with filtered backprojection and iterative methods. A 0.5 mm graphite rod was used to collect the forward model, while a truncated pseudoinverse was used to approximate the backward model for the iterative algorithm.
AB - Angular Domain Imaging (ADI) has been previously demonstrated to generate projection images of attenuating targets embedded within a turbid medium. The imaging system employs a silicon micro-tunnel array positioned between the sample and the detection system to reject scattered photons that have deviated from the initial propagation direction and to select for ballistic and quasi-ballistic photons that have retained their forward trajectory. Two dimensional tomographic images can be reconstructed from ADI projections collected at a multitude of angles. The objective of this work was to extend the system to three dimensions by collecting several tomographic images and stacking the reconstructed slices to generate a three dimensional volume representative of the imaging target. A diode laser (808nm, CW) with a beam expander was used to illuminate the sample cuvette. An Angular Filter Array (AFA) of 80 μm × 80 μm square-shaped tunnels 2 cm in length was used to select for image forming quasi-ballistic photons. Images were detected with a linear CCD. Our approach was to use a SCARA robot to rotate and translate the sample to collect sufficient projections to reconstruct a three dimensional volume. A custom designed 3D target consisting of 4 truncated cones was imaged and reconstructed with filtered backprojection and iterative methods. A 0.5 mm graphite rod was used to collect the forward model, while a truncated pseudoinverse was used to approximate the backward model for the iterative algorithm.
KW - 3D Optical Tomography
KW - Optical Image Reconstruction
KW - Optical Imaging
KW - Transmission Optical Imaging
UR - http://www.scopus.com/inward/record.url?scp=79955134758&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79955134758&partnerID=8YFLogxK
U2 - 10.1117/12.875828
DO - 10.1117/12.875828
M3 - Conference contribution
AN - SCOPUS:79955134758
SN - 9780819484345
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Optical Interactions with Tissue and Cells XXII
T2 - Optical Interactions with Tissue and Cells XXII
Y2 - 24 January 2011 through 26 January 2011
ER -