Three-dimensional angular domain optical projection tomography

Eldon Ng, Fartash Vasefi, Michael Roumeliotis, Bozena Kaminska, Jeffrey J.L. Carson

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Angular Domain Imaging (ADI) has been previously demonstrated to generate projection images of attenuating targets embedded within a turbid medium. The imaging system employs a silicon micro-tunnel array positioned between the sample and the detection system to reject scattered photons that have deviated from the initial propagation direction and to select for ballistic and quasi-ballistic photons that have retained their forward trajectory. Two dimensional tomographic images can be reconstructed from ADI projections collected at a multitude of angles. The objective of this work was to extend the system to three dimensions by collecting several tomographic images and stacking the reconstructed slices to generate a three dimensional volume representative of the imaging target. A diode laser (808nm, CW) with a beam expander was used to illuminate the sample cuvette. An Angular Filter Array (AFA) of 80 μm × 80 μm square-shaped tunnels 2 cm in length was used to select for image forming quasi-ballistic photons. Images were detected with a linear CCD. Our approach was to use a SCARA robot to rotate and translate the sample to collect sufficient projections to reconstruct a three dimensional volume. A custom designed 3D target consisting of 4 truncated cones was imaged and reconstructed with filtered backprojection and iterative methods. A 0.5 mm graphite rod was used to collect the forward model, while a truncated pseudoinverse was used to approximate the backward model for the iterative algorithm.

Original languageEnglish (US)
Title of host publicationOptical Interactions with Tissue and Cells XXII
DOIs
StatePublished - 2011
Externally publishedYes
EventOptical Interactions with Tissue and Cells XXII - San Francisco, CA, United States
Duration: Jan 24 2011Jan 26 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7897
ISSN (Print)1605-7422

Other

OtherOptical Interactions with Tissue and Cells XXII
Country/TerritoryUnited States
CitySan Francisco, CA
Period1/24/111/26/11

Keywords

  • 3D Optical Tomography
  • Optical Image Reconstruction
  • Optical Imaging
  • Transmission Optical Imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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