Thin-layer chromatography

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31 Scopus citations


TLC is used extensively in nucleic acid chemistry to monitor the progress of chemical reactions, to assay fractions collected from a larger chromatographic separation (e.g., column chromatography), and to determine optimal conditions prior to column chromatography. This unit describes methods for spotting test compounds onto a TLC plate, developing the plate in a suitable solvent system, visualizing the results, and calculating the retention factor (Rf). Candidate compounds can be co-spotted for identification without relying on Rf values.

Original languageEnglish (US)
Pages (from-to)A.3D.1-A.3D.13
JournalCurrent Protocols in Nucleic Acid Chemistry
Issue numberSUPPL. 34
StatePublished - 2008


  • Nucleosides
  • Nucleotides
  • Separation
  • TLC
  • Thin-layer chromatography

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry


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