The WNT7A/WNT7B/GPR124/RECK signaling module plays an essential role in mammalian limb development

Yanshu Wang, Arjun Venkatesh, Jiajia Xu, Mingxin Xu, John Williams, Philip M. Smallwood, Aaron James, Jeremy Nathans

Research output: Contribution to journalArticlepeer-review


In central nervous system vascular endothelial cells, signaling via the partially redundant ligands WNT7A and WNT7B requires two co-activator proteins, GPR124 and RECK. WNT7A and RECK have been shown previously to play a role in limb development, but the mechanism of RECK action in this context is unknown. The roles of WNT7B and GPR124 in limb development have not been investigated. Using combinations of conventional and/or conditional loss-of-function alleles for mouse Wnt7a, Wnt7b, Gpr124 and Reck, including a Reck allele that codes for a protein that is specifically defective in WNT7A/WNT7B signaling, we show that reductions in ligand and/or co-activator function synergize to cause reduced and dysmorphic limb bone growth. Two additional limb phenotypes – loss of distal Lmx1b expression and ectopic growth of nail-like structures – occur with reduced Wnt7a/Wnt7b gene copy number and, respectively, with Reck mutations and with combined Reck and Gpr124 mutations. A third limb phenotype – bleeding into a digit – occurs with the most severe combinations of Wnt7a/Wnt7b, Reck and Gpr124 mutations. These data imply that the WNT7A/WNT7BFRIZZLED-LRP5/LRP6-GPR124-RECK signaling system functions as an integral unit in limb development.

Original languageEnglish (US)
Article numberdev200340
JournalDevelopment (Cambridge)
Issue number9
StatePublished - May 2022


  • Blood-brain barrier
  • Limb development
  • Mouse
  • Wnt signaling

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology


Dive into the research topics of 'The WNT7A/WNT7B/GPR124/RECK signaling module plays an essential role in mammalian limb development'. Together they form a unique fingerprint.

Cite this