The Use of ECD/ETD to Identify the Site of Electrostatic Interaction in Noncovalent Complexes

Shelley N. Jackson, Sucharita Dutta, Amina S. Woods

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


Electrostatic interactions play an important role in the formation of noncovalent complexes. Our previous work has highlighted the role of certain amino acid residues, such as arginine, glutamate, aspartate, and phosphorylated/sulfated residues, in the formation of salt bridges resulting in noncovalent complexes between peptides. Tandem mass spectrometry (MS) studies of these complexes using collision-induced dissociation (CID) have provided information on their relative stability. However, product-ion spectra produced by CID have been unable to assign specifically the site of interaction for the complex. In this work, tandem MS experiments were conducted on noncovalent complexes using both electron capture dissociation (ECD) and electron-transfer dissociation (ETD). The resulting spectra were dominated by intramolecular fragments of the complex with the electrostatic interaction site intact. Based upon these data, we were able to assign the binding site for the peptides forming the noncovalent complex.

Original languageEnglish (US)
Pages (from-to)176-179
Number of pages4
JournalJournal of the American Society for Mass Spectrometry
Issue number2
StatePublished - Feb 2009
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy


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