The TLR9 agonist CpG fails to enhance the acquisition of Plasmodium falciparum-specific memory B cells in semi-immune adults in Mali

Boubacar Traore; Younoussou Koné; Safiatou Doumbo; Didier Doumtabé; Abdramane Traoré; Peter D. Crompton; Marko Mircetic; Chiung Yu Huang; Kassoum Kayentao; Alassane Dicko; Issaka Sagara; Ruth D. Ellis; Kazutoyo Miura; Agnes Guindo; Louis H. Miller; Ogobara K. Doumbo; Susan K. Pierce

doi:10.1016/j.vaccine.2009.08.023

The TLR9 agonist CpG fails to enhance the acquisition of Plasmodium falciparum-specific memory B cells in semi-immune adults in Mali

Boubacar Traore, Younoussou Koné, Safiatou Doumbo, Didier Doumtabé, Abdramane Traoré, Peter D. Crompton, Marko Mircetic, Chiung Yu Huang, Kassoum Kayentao, Alassane Dicko, Issaka Sagara, Ruth D. Ellis, Kazutoyo Miura, Agnes Guindo, Louis H. Miller, Ogobara K. Doumbo, Susan K. Pierce

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Antibodies play a key role in controlling blood stage malaria infections, and an effective blood stage malaria vaccine will likely require that it induce vaccine-specific memory B cells (MBCs). Our previous studies showed that the addition of the TLR9 agonist CpG to Plasmodium falciparum protein subunit vaccines greatly increased their efficacy in inducing MBCs in nonimmune U.S. volunteers. Here we show that in contrast the same CpG-containing malaria vaccine did not enhance the acquisition of MBCs in semi-immune adults living in Mali. Understanding the molecular basis of this apparent refractoriness to TLR9 agonist will be of significant interest in vaccine design.

Original language	English (US)
Pages (from-to)	7299-7303
Number of pages	5
Journal	Vaccine
Volume	27
Issue number	52
DOIs	https://doi.org/10.1016/j.vaccine.2009.08.023
State	Published - Dec 9 2009
Externally published	Yes

Keywords

Malaria
Memory B cells
Plasmodium falciparum
Toll-like receptor 9

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/j.vaccine.2009.08.023

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Traore, B., Koné, Y., Doumbo, S., Doumtabé, D., Traoré, A., Crompton, P. D., Mircetic, M., Huang, C. Y., Kayentao, K., Dicko, A., Sagara, I., Ellis, R. D., Miura, K., Guindo, A., Miller, L. H., Doumbo, O. K., & Pierce, S. K. (2009). The TLR9 agonist CpG fails to enhance the acquisition of Plasmodium falciparum-specific memory B cells in semi-immune adults in Mali. Vaccine, 27(52), 7299-7303. https://doi.org/10.1016/j.vaccine.2009.08.023

Traore, B, Koné, Y, Doumbo, S, Doumtabé, D, Traoré, A, Crompton, PD, Mircetic, M, Huang, CY, Kayentao, K, Dicko, A, Sagara, I, Ellis, RD, Miura, K, Guindo, A, Miller, LH, Doumbo, OK & Pierce, SK 2009, 'The TLR9 agonist CpG fails to enhance the acquisition of Plasmodium falciparum-specific memory B cells in semi-immune adults in Mali', Vaccine, vol. 27, no. 52, pp. 7299-7303. https://doi.org/10.1016/j.vaccine.2009.08.023

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title = "The TLR9 agonist CpG fails to enhance the acquisition of Plasmodium falciparum-specific memory B cells in semi-immune adults in Mali",

abstract = "Antibodies play a key role in controlling blood stage malaria infections, and an effective blood stage malaria vaccine will likely require that it induce vaccine-specific memory B cells (MBCs). Our previous studies showed that the addition of the TLR9 agonist CpG to Plasmodium falciparum protein subunit vaccines greatly increased their efficacy in inducing MBCs in nonimmune U.S. volunteers. Here we show that in contrast the same CpG-containing malaria vaccine did not enhance the acquisition of MBCs in semi-immune adults living in Mali. Understanding the molecular basis of this apparent refractoriness to TLR9 agonist will be of significant interest in vaccine design.",

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note = "Funding Information: This work was supported by the Intramural Research Program of the National Institute of Allergy and Infectious Diseases, National Institutes of Health. The authors are grateful for the gracious participation of the study volunteers of Donegoubougou and the diligent efforts of the members of the Malaria Vaccine Development Branch and the Malaria Research and Training Center clinical trial teams. Thanks to Coley Pharmaceuticals, a Pfizer Company, for supplying CPG 7909.",

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AU - Koné, Younoussou

AU - Doumbo, Safiatou

AU - Doumtabé, Didier

AU - Traoré, Abdramane

AU - Crompton, Peter D.

AU - Mircetic, Marko

AU - Huang, Chiung Yu

AU - Kayentao, Kassoum

AU - Dicko, Alassane

AU - Sagara, Issaka

AU - Ellis, Ruth D.

AU - Miura, Kazutoyo

AU - Guindo, Agnes

AU - Miller, Louis H.

AU - Doumbo, Ogobara K.

AU - Pierce, Susan K.

N1 - Funding Information: This work was supported by the Intramural Research Program of the National Institute of Allergy and Infectious Diseases, National Institutes of Health. The authors are grateful for the gracious participation of the study volunteers of Donegoubougou and the diligent efforts of the members of the Malaria Vaccine Development Branch and the Malaria Research and Training Center clinical trial teams. Thanks to Coley Pharmaceuticals, a Pfizer Company, for supplying CPG 7909.

PY - 2009/12/9

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N2 - Antibodies play a key role in controlling blood stage malaria infections, and an effective blood stage malaria vaccine will likely require that it induce vaccine-specific memory B cells (MBCs). Our previous studies showed that the addition of the TLR9 agonist CpG to Plasmodium falciparum protein subunit vaccines greatly increased their efficacy in inducing MBCs in nonimmune U.S. volunteers. Here we show that in contrast the same CpG-containing malaria vaccine did not enhance the acquisition of MBCs in semi-immune adults living in Mali. Understanding the molecular basis of this apparent refractoriness to TLR9 agonist will be of significant interest in vaccine design.

AB - Antibodies play a key role in controlling blood stage malaria infections, and an effective blood stage malaria vaccine will likely require that it induce vaccine-specific memory B cells (MBCs). Our previous studies showed that the addition of the TLR9 agonist CpG to Plasmodium falciparum protein subunit vaccines greatly increased their efficacy in inducing MBCs in nonimmune U.S. volunteers. Here we show that in contrast the same CpG-containing malaria vaccine did not enhance the acquisition of MBCs in semi-immune adults living in Mali. Understanding the molecular basis of this apparent refractoriness to TLR9 agonist will be of significant interest in vaccine design.

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The TLR9 agonist CpG fails to enhance the acquisition of Plasmodium falciparum-specific memory B cells in semi-immune adults in Mali

Abstract

Keywords

ASJC Scopus subject areas

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