The source of the nothing dehydrogenase activity in normal serum

Esteban Mezey; Kenneth C. Slater; Peter R. Holt

doi:10.1016/0009-8981(69)90219-8

The source of the nothing dehydrogenase activity in normal serum

Esteban Mezey, Kenneth C. Slater, Peter R. Holt

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The cause of the nothing dehydrogenase reaction observed when NAD⁺ is added to normal serum was investigated. The nothing dehydrogenase reaction was shown to have temperature inactivation properties, pH activity characteristics and kinetics comparable to those of the lactate dehydrogenase reaction. Furthermore, the reaction could no longer be detected after the removal of lactate or inhibition of lactate dehydrogenase. The nothing dehydrogenase reaction in serum appears to be due principally to the reduction of NAD+ by serum lactate dehydrogenase in the presence of endogenous serum lactate.

Original language	English (US)
Pages (from-to)	11-16
Number of pages	6
Journal	Clinica Chimica Acta
Volume	25
Issue number	1
DOIs	https://doi.org/10.1016/0009-8981(69)90219-8
State	Published - Jul 1969
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry
Biochemistry, medical

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10.1016/0009-8981(69)90219-8

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title = "The source of the nothing dehydrogenase activity in normal serum",

abstract = "The cause of the nothing dehydrogenase reaction observed when NAD+ is added to normal serum was investigated. The nothing dehydrogenase reaction was shown to have temperature inactivation properties, pH activity characteristics and kinetics comparable to those of the lactate dehydrogenase reaction. Furthermore, the reaction could no longer be detected after the removal of lactate or inhibition of lactate dehydrogenase. The nothing dehydrogenase reaction in serum appears to be due principally to the reduction of NAD+ by serum lactate dehydrogenase in the presence of endogenous serum lactate.",

author = "Esteban Mezey and Slater, {Kenneth C.} and Holt, {Peter R.}",

note = "Funding Information: + This investigation was supported in part by grants I Fz AM-33137-o1 and HE 10055 from the National Institutes of Health. * Norman Jolliffe fellow in clinical nutrition of Columbia University, formerly post-doctoral fellow of the National Institutes of Health. Present address, Johns Hopkins University School of Medicine, Department of Medicine, Baltimore, Maryland.",

year = "1969",

month = jul,

doi = "10.1016/0009-8981(69)90219-8",

language = "English (US)",

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journal = "Clinica Chimica Acta",

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T1 - The source of the nothing dehydrogenase activity in normal serum

AU - Mezey, Esteban

AU - Slater, Kenneth C.

AU - Holt, Peter R.

N1 - Funding Information: + This investigation was supported in part by grants I Fz AM-33137-o1 and HE 10055 from the National Institutes of Health. * Norman Jolliffe fellow in clinical nutrition of Columbia University, formerly post-doctoral fellow of the National Institutes of Health. Present address, Johns Hopkins University School of Medicine, Department of Medicine, Baltimore, Maryland.

PY - 1969/7

Y1 - 1969/7

N2 - The cause of the nothing dehydrogenase reaction observed when NAD+ is added to normal serum was investigated. The nothing dehydrogenase reaction was shown to have temperature inactivation properties, pH activity characteristics and kinetics comparable to those of the lactate dehydrogenase reaction. Furthermore, the reaction could no longer be detected after the removal of lactate or inhibition of lactate dehydrogenase. The nothing dehydrogenase reaction in serum appears to be due principally to the reduction of NAD+ by serum lactate dehydrogenase in the presence of endogenous serum lactate.

AB - The cause of the nothing dehydrogenase reaction observed when NAD+ is added to normal serum was investigated. The nothing dehydrogenase reaction was shown to have temperature inactivation properties, pH activity characteristics and kinetics comparable to those of the lactate dehydrogenase reaction. Furthermore, the reaction could no longer be detected after the removal of lactate or inhibition of lactate dehydrogenase. The nothing dehydrogenase reaction in serum appears to be due principally to the reduction of NAD+ by serum lactate dehydrogenase in the presence of endogenous serum lactate.

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JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

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The source of the nothing dehydrogenase activity in normal serum

Abstract

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