The source of the nothing dehydrogenase activity in normal serum

Esteban Mezey, Kenneth C. Slater, Peter R. Holt

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


The cause of the nothing dehydrogenase reaction observed when NAD+ is added to normal serum was investigated. The nothing dehydrogenase reaction was shown to have temperature inactivation properties, pH activity characteristics and kinetics comparable to those of the lactate dehydrogenase reaction. Furthermore, the reaction could no longer be detected after the removal of lactate or inhibition of lactate dehydrogenase. The nothing dehydrogenase reaction in serum appears to be due principally to the reduction of NAD+ by serum lactate dehydrogenase in the presence of endogenous serum lactate.

Original languageEnglish (US)
Pages (from-to)11-16
Number of pages6
JournalClinica Chimica Acta
Issue number1
StatePublished - Jul 1969
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical


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