Abstract
The cause of the nothing dehydrogenase reaction observed when NAD+ is added to normal serum was investigated. The nothing dehydrogenase reaction was shown to have temperature inactivation properties, pH activity characteristics and kinetics comparable to those of the lactate dehydrogenase reaction. Furthermore, the reaction could no longer be detected after the removal of lactate or inhibition of lactate dehydrogenase. The nothing dehydrogenase reaction in serum appears to be due principally to the reduction of NAD+ by serum lactate dehydrogenase in the presence of endogenous serum lactate.
Original language | English (US) |
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Pages (from-to) | 11-16 |
Number of pages | 6 |
Journal | Clinica Chimica Acta |
Volume | 25 |
Issue number | 1 |
DOIs | |
State | Published - Jul 1969 |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Clinical Biochemistry
- Biochemistry, medical