TY - JOUR
T1 - The sevenless+ protein is expressed apically in cell membranes of developing Drosophila retina; it is not restricted to cell R7
AU - Banerjee, Utpal
AU - Renfranz, Patricia J.
AU - Hinton, David R.
AU - Rabin, Bruce A.
AU - Benzer, Seymour
N1 - Funding Information:
We are grateful to Ft. Young, J. Mao, M. Anderson, and E. Eichenberger for excellent technical assistance. We thank J. Ella&s and M. Pickford for their technical advice and use of their EM facililies (funded by NIH ET03040-08), D. Ballinger and R. Hackett for many helpful suggestions and advice, and members of the Benzer lab for their review of the manuscript. This work was supported by a grant to S. B. (DCB-8409366) from the National Science Foundation and the :Markey Developmental Biology Program. U. B. is a Burroughs Welllcome Fund Fellow of the Life Sciences Research Foundation. R J. R. was supported in part by a National Research Service Award (1 T32GM07616) from the National Institute of General Medical Sciences. D. R. H. was supported by the Medical Research Council of Canada and the Gordon Ross Foundation. B. A. R. was supported by a Weizmann Fellowship.
PY - 1987/10/9
Y1 - 1987/10/9
N2 - In the sevenless (sev) mutants of Drosophila, a single cell type, photoreceptor R7, does not develop. We made monoclonal antibody against a sev+-β-galactosidase fusion protein, and used it to determine the ultrastructural localization of the sev+ protein in the larval eye disc. The protein is expressed on the apical surface of the developing retina. It is not restricted to cell R7; it is expressed in all the presumptive photoreceptor cells, cone cells, and possibly others. The protein localizes to the cell membranes of the apical tips and their microvilli, away from the bulk of the cell-cell contacts. Possible mechanisms for generating the specificity of the sev phenotype are discussed in light of these results.
AB - In the sevenless (sev) mutants of Drosophila, a single cell type, photoreceptor R7, does not develop. We made monoclonal antibody against a sev+-β-galactosidase fusion protein, and used it to determine the ultrastructural localization of the sev+ protein in the larval eye disc. The protein is expressed on the apical surface of the developing retina. It is not restricted to cell R7; it is expressed in all the presumptive photoreceptor cells, cone cells, and possibly others. The protein localizes to the cell membranes of the apical tips and their microvilli, away from the bulk of the cell-cell contacts. Possible mechanisms for generating the specificity of the sev phenotype are discussed in light of these results.
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U2 - 10.1016/0092-8674(87)90020-1
DO - 10.1016/0092-8674(87)90020-1
M3 - Article
C2 - 3115594
AN - SCOPUS:0023649247
SN - 0092-8674
VL - 51
SP - 151
EP - 158
JO - Cell
JF - Cell
IS - 1
ER -