The scorpion toxin Tf2 from Tityus fasciolatus promotes Nav1.3 opening

Thalita S. Camargos; Frank Bosmans; Solange C. Rego; Caroline B.F. Mourão; Elisabeth F. Schwartz

doi:10.1371/journal.pone.0128578

The scorpion toxin Tf2 from Tityus fasciolatus promotes Na_v1.3 opening

Thalita S. Camargos, Frank Bosmans, Solange C. Rego, Caroline B.F. Mourão, Elisabeth F. Schwartz

School of Medicine

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

We identified Tf2, the first β-scorpion toxin from the venom of the Brazilian scorpion Tityus fasciolatus. Tf2 is identical to Tb2-II found in Tityus bahiensis. We found that Tf2 selectively activates human (h)Na_v1.3, a neuronal voltage-gated sodium (Na_v) subtype implicated in epilepsy and nociception. Tf2 shifts hNa_v1.3 activation voltage to more negative values, thereby opening the channel at resting membrane potentials. Seven other tested mammalian Na_v channels (Na_v1.1-1.2; Na_v1.4-1.8) expressed in Xenopus oocytes are insensitive upon application of 1 μM Tf2. Therefore, the identification of Tf2 represents a unique addition to the repertoire of animal toxins that can be used to investigate Na_v channel function.

Original language	English (US)
Article number	e0128578
Journal	PloS one
Volume	10
Issue number	6
DOIs	https://doi.org/10.1371/journal.pone.0128578
State	Published - Jun 17 2015

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
General

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title = "The scorpion toxin Tf2 from Tityus fasciolatus promotes Nav1.3 opening",

abstract = "We identified Tf2, the first β-scorpion toxin from the venom of the Brazilian scorpion Tityus fasciolatus. Tf2 is identical to Tb2-II found in Tityus bahiensis. We found that Tf2 selectively activates human (h)Nav1.3, a neuronal voltage-gated sodium (Nav) subtype implicated in epilepsy and nociception. Tf2 shifts hNav1.3 activation voltage to more negative values, thereby opening the channel at resting membrane potentials. Seven other tested mammalian Nav channels (Nav1.1-1.2; Nav1.4-1.8) expressed in Xenopus oocytes are insensitive upon application of 1 μM Tf2. Therefore, the identification of Tf2 represents a unique addition to the repertoire of animal toxins that can be used to investigate Nav channel function.",

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AU - Mourão, Caroline B.F.

AU - Schwartz, Elisabeth F.

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N2 - We identified Tf2, the first β-scorpion toxin from the venom of the Brazilian scorpion Tityus fasciolatus. Tf2 is identical to Tb2-II found in Tityus bahiensis. We found that Tf2 selectively activates human (h)Nav1.3, a neuronal voltage-gated sodium (Nav) subtype implicated in epilepsy and nociception. Tf2 shifts hNav1.3 activation voltage to more negative values, thereby opening the channel at resting membrane potentials. Seven other tested mammalian Nav channels (Nav1.1-1.2; Nav1.4-1.8) expressed in Xenopus oocytes are insensitive upon application of 1 μM Tf2. Therefore, the identification of Tf2 represents a unique addition to the repertoire of animal toxins that can be used to investigate Nav channel function.

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The scorpion toxin Tf2 from Tityus fasciolatus promotes Na_v1.3 opening

Abstract

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